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采用血小板计数、巨核细胞形态观察及计数、巨核细胞集落培养技术，观察从人尿中纯化的天然血小板生成素（TPO）对Balb／c，h鼠巨核细胞生成及血小板产生的作用。小鼠随机分四组，Ⅰ组为对照组，Ⅱ、Ⅲ、Ⅳ组为TPO低剂量组（10U／R）中剂量组（50U／只）和高剂量组（100U／只），结果显示TPO能显著提高外周血液中的血小板数（P＜0.01）和骨髓中的巨核细胞数（P＜0.01），且有巨核细胞的脑体和胞核的显著增大的作用，在体外对巨核细胞集落形成有明显增加作用（P＜0.01）。这些结果表明从人尿中纯化的天然TPO具有促进巨核细胞增殖、分化、成熟及产生血小板的作用为TPO进入临床试验提供了依据。
Platelet count, morphology and count of megakaryocytes, megakaryocyte colony culture technique were used to observe the effect of TPO purified from human urine on megakaryocyte production and platelet production in Balb / c mice. The mice were randomly divided into four groups: control group Ⅰ, group Ⅱ, Ⅲ, Ⅳ TPO low dose group (10U / R) and high dose group (50U / (P <0.01) and the number of megakaryocytes in bone marrow (P <0.01), and significantly increased the number of megakaryocytes in the brain and nucleus. In vitro, the number of megakaryocyte colonies Formed a significant increase in the role (P <0.01). These results indicate that the purification of native TPO from human urine with the promotion of megakaryocyte proliferation, differentiation, maturation and the role of platelets provide a basis for clinical trials of TPO.