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一、概况在分析化学领域中,分光光度测定法是一种应用范围很广的方法.但是由于传统的单波长测定法要求试液本身透明,不能有浑浊,因而当试液在测定过程中慢慢产生浑浊时,就无法正确测定.单波长测定法对于吸收峰相互重叠的组份或背景很深的试样,也难于得到正确的结果.此外,在传统的单波长测定法中,由于试样池和参比池之间不匹配,试液与参比液组成不一致,以及对于弱的光信号如A<0.01的测定,均会产生较大的误差.
I. Overview In the field of analytical chemistry, spectrophotometry is a widely used method.But due to the traditional single wavelength measurement requires the test solution itself is transparent, can not be cloudy, so when the test solution in the determination of the slow When turbidity is generated slowly, it is impossible to measure correctly.Under the single wavelength measurement method, it is difficult to obtain correct results for components having a large absorption peak or samples having a large background, and in the conventional single wavelength measurement method, Mismatch between the sample pool and the reference pool, inconsistencies in the composition of the test solution and the reference solution, and large errors in the determination of a weak optical signal such as A <0.01.