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目的探讨正常宫颈和宫颈癌组织中钙激活性中电导钾离子通道(IKCa1)与人类宫颈鳞状上皮细胞癌的相关性。方法取2007年10月至2008年4月泸州医学院附属医院手术治疗的30例不同分化程度的宫颈鳞状上皮细胞癌患者病变部位组织作为实验组,同时期18例正常宫颈上皮组织作为对照组,分别采用免疫组织化学方法检测上述组织中IKCa1蛋白产物表达部位及表达水平。结果 IKCa1在30例宫颈鳞状上皮细胞癌组织细胞核及细胞膜上均呈阳性表达,阳性表达率为100.0%;18例正常宫颈上皮组织中仅1例在鳞状上皮部位细胞膜部位有较弱表达,阳性表达率为5.6%,IKCa1蛋白产物在宫颈鳞状上皮细胞癌组织中的表达程度及表达部位均与正常宫颈组织中存在明显差异(Z=-6.109,P=0.00,P<0.05)。在不同分化程度的宫颈鳞状上皮细胞癌组织中,高分化鳞癌组阳性表达率为40.0%,中分化鳞癌组阳性率为90.0%,低分化鳞癌组阳性率为100.0%;IK-Ca1蛋白产物表达程度与癌细胞分化程度呈负相关(χ2=8.4,P<0.05)。结论宫颈鳞状上皮细胞癌组织中IK-Ca1蛋白产物的表达明显高于正常宫颈组织;且IKCa1蛋白产物表达水平与癌细胞的分化程度呈负相关;过高表达的IKCa1与宫颈鳞状上皮细胞癌的发生和发展可能存在重要联系。
Objective To investigate the correlation between calcium-activated potassium channel (IKCa1) and human cervical squamous cell carcinoma in normal cervical and cervical cancer tissues. Methods From October 2007 to April 2008, 30 cases of squamous cell carcinoma of the squamous cell carcinoma of the cervix underwent surgery in Luzhou Medical College Affiliated Hospital. Twenty-eight normal cervical epithelial tissues were used as the control group Immunohistochemistry was used to detect the expression of IKCa1 protein in the above tissues. Results The expression of IKCa1 in the nuclei and cell membrane of 30 cases of cervical squamous cell carcinoma was positive, the positive rate was 100.0%. Only 1 of the 18 cases of normal cervical epithelium had weak expression in the cell membrane of squamous epithelium, The positive expression rate of IKCa1 protein in cervical squamous cell carcinoma tissues was 5.6%. The expression level of IKCa1 protein in cervical squamous cell carcinoma tissues was significantly different from that in normal cervical tissues (Z = -6.109, P = 0.00, P <0.05). In differentiated cervical squamous cell carcinoma tissues, the positive rate was 40.0% in well-differentiated squamous cell carcinoma, 90.0% in moderately differentiated squamous cell carcinoma, and 100.0% in poorly differentiated squamous cell carcinoma. The positive rate of IK- The expression level of Ca1 protein was negatively correlated with the degree of cancer cell differentiation (χ2 = 8.4, P <0.05). Conclusion The expression of IK-Ca1 protein in cervical squamous cell carcinoma tissues is significantly higher than that in normal cervical tissues. The expression level of IKCa1 protein is negatively correlated with the differentiation of cancer cells. The overexpression of IKCa1 and cervical squamous epithelial cells There may be important links between the occurrence and development of cancer.