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目的构建新型甲胎蛋白安培免疫传感器。方法首先在玻碳电极(GCE)表面修饰一层羧基化碳纳米管(CNTs),然后利用带负电荷的DNA分子和带正电荷的硫堇之间的静电作用,层层自组装修饰硫堇以增强检测信号,然后利用硫堇的氨基固定纳米金,以便固定抗体,最后利用牛血清白蛋白封闭未结合位点。结果修饰的碳纳米管能够显著地提高电极的导电性,利用层层组装技术修饰了5层硫堇。在优化的条件下(pH7.0,温浴时间25min),制作的甲胎蛋白免疫传感器线性范围在0.5～25ng/ml内,检测限0.02ng/ml。结论成功利用层层自组装技术构建新型基于碳纳米管修饰的无酶型甲胎蛋白安培免疫传感器,该传感器灵敏度高,特异性好,有望成为原发性肝癌早期诊断的新方法。
Objective To construct a new type of AFP immunosensor. Methods Firstly, a series of carboxylated carbon nanotubes (CNTs) were modified on the surface of glassy carbon electrode (GCE), and then the electrostatic interaction between negatively charged DNA molecules and positively charged thionine In order to enhance the detection signal, and then use thionine amino fixed nano gold in order to immobilize the antibody, and finally the use of bovine serum albumin unbound site. Results Modified carbon nanotubes (CNTs) could significantly improve the conductivity of the electrodes. Five layers of thionine were modified by the layer assembly technique. Under the optimal conditions (pH7.0, 25min incubation time), the linear range of the immunofluorescent protein was 0.5 ~ 25ng / ml and the detection limit was 0.02ng / ml. Conclusion A new type of amylase immunosensor based on carbon nanotubes successfully constructed with multi-layer self-assembly technique has been developed. This sensor has the advantages of high sensitivity and specificity and is expected to become a new method for the early diagnosis of primary liver cancer.