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目的:建立高效液相色谱法同时测定止咳宁嗽胶囊中的有效成分绿原酸和橙皮苷的方法。方法:样品用甲醇超声提取,在ZorbaxSB-C18(3.0mm×250mm,5μm)色谱柱上分离,以含1%乙酸的水(A)-乙腈(B)体系为流动相进行阶梯洗脱,程序为0min(82∶18)～5min(82∶18)～5.1min(75∶25)～20min(75∶25);流速为0.35mL·min-1;327nm紫外检测。结果:绿原酸在进样量为0.4～10.0μg·mL-1内呈良好的线性关系;橙皮苷在进样量为6.2～155.0μg·mL-1内呈良好的线性关系。绿原酸的平均回收率为99.2%,RSD为1.0%。橙皮苷的平均回收率为99.6%,RSD为0.9%。结论:该方法简便快速,结果准确,重复性好,可为止咳宁嗽胶囊的质量控制提供参考依据。
Objective: To establish a method for the simultaneous determination of chlorogenic acid and hesperidin in Zhike NingChao Capsules by high performance liquid chromatography. Methods: The sample was extracted with methanol and separated on a Zorbax SB-C18 column (3.0 mm × 250 mm, 5 μm). The mobile phase was eluted with 1% acetic acid in water (A) (82:18) ~ 5min (82:18) ~ 5.1min (75:25) ~ 20min (75:25); the flow rate was 0.35mL · min-1; 327nm UV detection. Results: There was a good linear relationship between chlorogenic acid and injection volume of 0.4 ~ 10.0μg · mL-1. Hesperidin had a good linear relationship with injection volume of 6.2 ~ 155.0μg · mL-1. The average recovery of chlorogenic acid was 99.2% with a RSD of 1.0%. The average recovery of hesperidin was 99.6% with a RSD of 0.9%. Conclusion: The method is simple, rapid, accurate and reproducible. It can provide a reference for the quality control of Cough Capsule.