论文部分内容阅读
以我国目前生产中广泛应用的杂交稻恢复系蜀恢527和明恢86为轮回亲本,以江西丝苗为供体亲本配制BC2F2混合群体。通过逐代人工接种筛选,获得49个BC2F4抗病导入系。对抗病选择导入系进行基因型分析,利用基于遗传搭车原理的卡方检验对等位基因导入频率的偏离进行检测,共检测到12个显著位点,2个群体定位的QTL各有1个在以往不同群体中也能被检测到。主要农艺性状分析表明,非接种条件下,选择导入系群体的抽穗期、株高和千粒重等与轮回亲本无显著差异;在接种的条件下,在2个试验地点中,分别有6个和2个蜀恢527和明恢86背景的株系抗病性显著高于轮回亲本,产量与轮回亲本无显著差异。上述结果为水稻抗纹枯病分子育种提供了有用的信息和中间材料。
The hybrid rice restorer lines Shuhui 527 and Minghui 86 which are widely used in the present production of our country are the recurrent parents and the BC2F2 mixed population is prepared from the Jiangxi silkworm seedlings as the donor parent. Forty-nine BC2F4 resistant lines were obtained by artificial inoculation screening. A total of 12 significant loci were detected for the frequency of allele import using the chi-square test based on the genetic algorithm of ride-on. One QTL was located in each of the two populations In the past, different groups can also be detected. The main agronomic traits analysis showed that under non-inoculation conditions, the heading date, plant height and 1000-grain weight of the selected inbred lines were not significantly different from those of the recurrent parent. Under the conditions of inoculation, there were 6 and 2 The resistance of Shuhui 527 and Minghui 86 backgrounds was significantly higher than that of the recurrent parent, and no significant difference was found between the yield and the recurrent parent. The above results provide useful information and intermediate materials for the molecular breeding of rice sheath blight resistance.