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目的探讨简单有效的兔心肌缺血再灌注损伤模型制备方法,并利用心肌声学造影评价兔心肌缺血再灌注损伤。方法将70只日本大耳白兔随机分为2组:35只心肌缺血/再灌注组(I/R)和35只假手术组(SH)。I/R组阻断冠状动脉左前降支90min,再灌注60、120、180min及1周;SH组开胸后同一部位只穿线不阻断。观察两组兔术前及术后各时段心肌声学造影的变化,最后行HE及MASSON染色。结果成功制备兔心肌缺血再灌注损伤模型30只,I/R组缺血90min至再灌注180min兔损伤节段造影剂视频强度超声均值对比术前及SH组均有不同程度减低,且差异有统计学意义(P<0.05)。I/R组兔术后HE及MASSON染色均证实有心肌损伤病理表现,随着早期再灌注时间的延长损伤程度逐渐加重。结论通过阻断兔冠状动脉左前降支90min后解除阻断使其再灌注可成功建立心肌缺血再灌注损伤模型,心肌声学造影可以有效评价心肌缺血再灌注损伤。
Objective To investigate a simple and effective method for the preparation of rabbit model of myocardial ischemia-reperfusion injury and to evaluate the myocardial ischemia-reperfusion injury by myocardial contrast echocardiography. Methods Seventy Japanese white rabbits were randomly divided into 2 groups: 35 I / R rats and 35 sham operation rats. The I / R group blocked the left anterior descending coronary artery 90min, reperfusion 60,120,180min and 1 week; The changes of myocardial contrast echocardiography before and after operation were observed in both groups. HE staining and MASSON staining were performed. Results 30 rabbits with myocardial ischemia / reperfusion injury were successfully established. The mean ultrasound intensity of video intensities in rabbits with I / R group after 90 min of ischemia and 180 min of reperfusion was lower than that before operation and in SH group, and the difference was Statistical significance (P <0.05). I / R group after rabbit HE and MASSON staining confirmed the pathological findings of myocardial injury, with the early reperfusion time increases the degree of injury gradually increased. Conclusion Myocardial ischemia-reperfusion injury can be successfully established by blocking the left anterior descending coronary artery of rabbit for 90 minutes after being unblocked and then reperfused. Myocardial contrast-enhanced ultrasound can be used to evaluate myocardial ischemia-reperfusion injury.