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【目的】观察全反式维甲酸 (ATRA)、5 FU单用和合用对胃癌细胞端粒酶活性的影响及其抗癌作用。【方法】A TRA、5 FU单用和合用处理体外培养的胃癌MGC 80 3细胞和荷胃癌裸鼠 ,用MTT法测定细胞活力 ,测量裸鼠用药前后的瘤体积 ,用端粒重复序列扩增法测定端粒酶活性。【结果】随着ATRA、5 FU浓度增高 ,作用时间延长 ,胃癌细胞活力逐渐下降。40 μmol/LATRA组、5 μmol/L 5 FU组和合用组 (AF组 ,40 μmol/LATRA加 5 μmol/L 5 FU)处理胃癌细胞 3d细胞活力分别为 46 %、47%和 8% (P <0 0 1) ,端粒酶活性分别为 45 7% (P <0 0 1)、10 0 %和 46 1% (P <0 0 1)。用药后ATRA组、合用组 (AF组 )瘤体积显著小于溶剂对照组 (不加药 ) ,5 FU组瘤体积显著小于对照组 ,用药后ATRA组、5 FU组、合用组端粒酶活性分别为 6 1%、10 0 %和 6 3 %。【结论】ATRA、5 FU在体外、体内均能显著抑制胃癌细胞的生长 ,二者合用对体外培养的胃癌细胞生长有协同抑制作用。抑制胃癌细胞端粒酶活性可能是ATRA抗肿瘤效应的机制之一。
【Aim】 To observe the effect of ATRA and 5 FU alone or in combination on telomerase activity and its anticancer activity in gastric cancer cells. 【Method】 ATRA and 5 FU alone and in combination were used to treat gastric cancer MGC 80 3 cells and gastric cancer-bearing nude mice cultured in vitro. The cell viability was measured by MTT assay. The tumor volumes of nude mice before and after treatment were measured and amplified by telomeric repeat amplification Determination of telomerase activity. 【Results】 With the increase of ATRA and 5 FU concentrations and the prolonged action time, the viability of gastric cancer cells gradually decreased. The viability of 3d cells in gastric cancer cells treated with 40 μmol / L ATRA, 5 μmol / L 5 FU and combination groups (AF, 40 μmol / L ATRA plus 5 μmol / L 5 FU) were 46%, 47% and 8% <0 0 1). The telomerase activity was 45 7% (P 0 01), 100% and 46 1% respectively (P 0 01). The tumor volumes in ATRA group and combined group (AF group) were significantly smaller than those in solvent control group (without drug addition), and the tumor volume in 5 FU group was significantly smaller than that in control group. The telomerase activity in ATRA group, 5 FU group and combined group were 6 1%, 100% and 63% respectively. 【Conclusion】 ATRA and 5 FU can significantly inhibit the growth of gastric cancer cells both in vitro and in vivo, and the combination of both can inhibit the growth of gastric cancer cells in vitro. Inhibition of telomerase activity in gastric cancer cells may be one of the mechanisms of anti-tumor effect of ATRA.