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Using the tetrazolium (MTT) assay, we examined the cytotoxicities of recombinant human tumor necrosis factor (rhTNF) and five chemotherapeutic agents, namely CTX, 5-FU, VCR, DDP and KSM, on human ovarian cancer cell lines OVCAR_3 and CAOV_3. The results showed that the cytotoxicities of rhTNF at concentrations of 50-50000 U / mi on OVCAR_3 cell line and CAOV_3 cell line exposed to rhTNF for 24 hours were from 14.2% ±6.8% to 67.2%± 3.00 and from 8.2%±4.3% to 60.9%±1.3%, respectively. The cytotoxicities of all five chemotherapeutic agents tested on the two cell lines were much lower than that of rhTNF. We also studied the combined antitumor potential of rhTNF with the five chemotherapeutic agents and the results showed that there were various degrees of synergism in cytotoxicities of rhTNF in combination with DDP or KSM on the two cell lines. Based on experiments in vitro, the in vivo antitumor activities of rhTNF, both alone and in combination with KSM, were examined in OVCAR_3 cancer transplanted in
Using the tetrazolium (MTT) assay, we examined the cytotoxicities of recombinant human tumor necrosis factor (rhTNF) and five chemotherapeutic agents, namely CTX, 5-FU, VCR, DDP and KSM, on human ovarian cancer cell lines OVCAR_3 and CAOV_3. results showed that the cytotoxicities of rhTNF at concentrations of 50-50000 U / mi on OVCAR_3 cell line and CAOV_3 cell line exposed to rhTNF for 24 hours were from 14.2% ± 6.8% to 67.2% ± 3.00 and from 8.2% ± 4.3% to 60.9% ± 1.3%, respectively. The cytotoxicities of all five chemotherapeutic agents tested on the two cell lines were much lower than that of rhTNF. We also studied the combined antitumor potential of rhTNF with the five chemotherapeutic agents and the results showed that there were each degrees of synergism in cytotoxicities of rhTNF in combination with DDP or KSM on the two cell lines. Based on experiments in vitro, the in vivo antitumor activities of rhTNF, both alone and in combination with KSM, were examined in OVCAR_3 canc er transplanted in