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目的了解珠三角地区吸毒人群丙型肝炎病毒(HCV)亚型的种类和组成。方法根据珠三角地区9个地级市艾滋病吸毒哨点的HCV患病率计算最低样本量,对吸毒哨点2014-2015年HCV抗体阳性样本进行系统抽样,得样本455份。通过提取样本RNA、PCR扩增、基因片段测序、构建不同片段进化树来确定亚型,并对其流行病学资料进行统计分析。结果 455份样本中,亚型及比重为1a(2.4%)、1b(7.0%)、2a(0.2%)、3a(16.9%)、3b(10.8%)、6a(61.5%)、6e(0.9%)、6k(0.2%)。亚型在年龄(χ2=30.798,P<0.05)、户籍所在地(χ2=12.924,P<0.05)、民族(χ2=21.432,P<0.05)的分布差异有统计学意义。6e与广西参考株有78%可靠性。1b、3a、6a分支下有小型进化簇。结论珠三角的吸毒人群中,6a是主要亚型,6e由广西传入。各亚型感染来源一致,存在小范围跨市传播情况。
Objective To understand the type and composition of hepatitis C virus (HCV) subtype in addicts in the Pearl River Delta Region. Methods According to the lowest prevalence of HCV prevalence in HIV / AIDS sentinel sites of 9 prefecture-level cities in the Pearl River Delta region, 455 samples of HCV antibody positive samples from 2014 to 2015 were systematically sampled. By extracting the sample RNA, PCR amplification, sequencing of gene fragments, constructing different fragments phylogenetic tree to determine subtypes, and its epidemiological data for statistical analysis. Results Among the 455 samples, the subtype and the specific gravity were 1a (2.4%), 1b (7.0%), 2a (0.2%), 3a (16.9%), 3b (10.8%), 6a %), 6k (0.2%). The distribution of subtypes was statistically significant at age (χ2 = 30.798, P <0.05), where the household was located (χ2 = 12.924, P <0.05) and ethnicity (χ2 = 21.432, P <0.05). 6e and Guangxi reference strains have 78% reliability. 1b, 3a, 6a branches of small evolutionary cluster. Conclusion Among the drug users in the Pearl River Delta, 6a is the major subtype and 6e is introduced from Guangxi. The sources of infection in each subtype are the same, with small-scale cross-market transmission.