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目的探讨肝脏药物转运体(solute carrier organic anion transporter family member 1B1,SLCO1B1)和多药耐药性蛋白(ATP-binding cassette subfamily B member 1,ABCB1)基因多态性与汉族人群抗结核药物性肝损伤的关系。方法采用1∶1配对的病例对照研究设计,选择抗结核治疗致肝损伤者171例为病例组,无肝损伤者171例为对照组。应用聚合酶链反应-限制性片段长度多态性分析(polymerase chain reaction and restriction fragmentlength polymorphism,PCR-RFLP)技术,检测171对肺结核病患者SLCO1B1基因388A/G位点和ABCB1基因1236C/T位点多态性情况。采用SPSS 13.0统计软件进行单因素分析。结果 SLCO1B1基因388A/G位点的A、G等位基因频率在病例组为22.8%和77.2%,在对照组分别为25.7%和74.3%;ABCB1基因1236C/T位点的C、T等位基因频率在病例组和对照组相同,均为28.4%和71.6%。单因素分析结果表明,SLCO1B1和ABCB1基因的多态性分布在病例组与对照组间差异均无统计学意义(均有P>0.05),与肝细胞型肝损伤的发生无统计学关系(P>0.05)。结论 SLCO1B1-388A/G和ABCB1-1236C/T基因多态性可能与抗结核药物性肝损伤、肝细胞型肝损伤的发生无相关性。
Objective To investigate the relationship between the gene polymorphisms of liver cirrhosis (SLCO1B1) and multi-drug resistance protein (ABCB1) in Han population and anti-TB drug-induced liver injury Relationship. Methods A case-control study was designed with a 1: 1 pairing. A total of 171 cases of liver damage caused by anti-TB treatment were selected as the case group, and 171 cases without liver injury were selected as the control group. 171 SBCO1B1 388A / G and ABCB1 1236C / T were detected in 171 pairs of patients with pulmonary tuberculosis by PCR-RFLP. Polymorphism. Univariate analysis using SPSS 13.0 statistical software. Results The frequencies of A and G alleles in the 388A / G site of SLCO1B1 gene were 22.8% and 77.2% in the case group and 25.7% and 74.3% in the control group respectively. The C and T alleles of the 1236C / T site of ABCB1 gene Gene frequency in the case group and control group the same, both 28.4% and 71.6%. Univariate analysis showed that there was no significant difference in the distribution of SLCO1B1 and ABCB1 among the cases and controls (P> 0.05), but not with the incidence of hepatocellular hepatocellular injury (P > 0.05). Conclusion The polymorphisms of SLCO1B1-388A / G and ABCB1-1236C / T may not be associated with anti-TB drug-induced liver injury and hepatocellular liver injury.