论文部分内容阅读
检测miR-106a在淋巴瘤Jurkat细胞中的表达及阿霉素对其表达的影响,探讨miR-106a在淋巴瘤发病中的作用机理。采用淋巴瘤Jurkat细胞,用不同浓度阿霉素处理,以正常人外周血单个核细胞作对照,应用实时定量PCR(Real-Time PCR)方法检测上述各组miR-106a中的表达情况;采用MTT方法检测Jurkat细胞增殖情况;采用流式细胞术(FCM)分析阿霉素干预后Jurkat细胞凋亡及周期变化。淋巴瘤Jurkat细胞中miR-106a表达水平明显高于正常对照组(t=10.27,p<0.01)。用不同浓度阿霉素作用于Jurkat细胞48 h、72 h后,miR-106a表达水平较对照组均下降,并随阿霉素浓度升高其相对表达量降低(48 h F=198.57,72 h F=495.43,p均<0.01);对Jurkat细胞的增殖抑制率(48 h F=65.54,72 h F=110.08,p均<0.01)和凋亡率均高于对照组(48 h F=1072.19,72 h F=3 271.66,p均<0.01)。miR-106a在淋巴瘤Jurkat细胞中的表达较正常人增高,阿霉素对其表达有抑制作用,miR-106a可能与淋巴瘤的发生发展有关。
To detect the expression of miR-106a in lymphoma Jurkat cells and doxorubicin on the expression of miR-106a in the pathogenesis of lymphoma. Using lymphoma Jurkat cells with different concentrations of doxorubicin treatment, normal human peripheral blood mononuclear cells as a control, real-time PCR (Real-Time PCR) method to detect the above groups of miR-106a expression; using MTT Methods The proliferation of Jurkat cells was detected by flow cytometry (FCM). The apoptosis and the cycle changes of Jurkat cells were analyzed by flow cytometry (FCM). The expression of miR-106a in lymphoma Jurkat cells was significantly higher than that in the normal control group (t = 10.27, p <0.01). The expression of miR-106a in Jurkat cells treated with different concentrations of doxorubicin for 48 h and 72 h decreased compared with the control group, and decreased with the increase of doxorubicin concentration (48 h F = 198.57 and 72 h (48 h F = 65.54, 72 h F = 110.08, p <0.01) and the apoptosis rate of Jurkat cells were higher than that of the control group (F = 495.43, p <0.01) , 72 h F = 3 271.66, p <0.01). miR-106a expression in lymphoma Jurkat cells increased compared with normal, doxorubicin inhibited its expression, miR-106a may be related to the occurrence and development of lymphoma.