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效应性CD8+T细胞分化成熟是机体抵抗病原体侵袭及疫苗发挥作用的重要环节。激活后数小时内,初始CD8+T细胞起始一系列基因转录促进向效应性及记忆型T细胞分化,然而这一过程的调节机制尚不清楚。利用病毒转导shRNA是一项经典的通过特异性敲低某基因实现研究该基因功能的技术,但该项技术在初始T细胞中效率非常低。后续改进的方法多集中在通过给予各种体外刺激如TCR、感染、细胞因子等激活初始T细胞而达到提高转导率的目的,可是这种额外的干扰对细胞的正常分化过程造成较大影响。
Differentiation and maturation of effector CD8 + T cells is an important part of the body against the invasion of pathogens and the role of the vaccine. Within a few hours after activation, a sequence of gene transcription initiated by naive CD8 + T cells promotes effector and memory T cell differentiation, however, the regulatory mechanism of this process is not clear. The use of viruses to transduce shRNAs is a classic technique for studying the function of a gene by specifically knocking down a gene, but the technique is very inefficient in naive T cells. Subsequent improvements have focused on achieving the goal of increasing transduction by activating primary T cells by administering various in vitro stimuli such as TCRs, infections, cytokines, etc. However, such additional interference has a major impact on the normal differentiation of cells .