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目的:分析B7-H1及Bcl-2蛋白在人结直肠癌中的表达,并探讨其与结直肠癌临床病理特征之间的关系。方法:用免疫组织化学法检测57例手术切除的结直肠癌组织标本及癌旁正常组织标本中B7-H1及Bcl-2的表达,并结合临床病理特征进行相关性分析。结果:与癌旁正常组织相比,B7-H1及Bcl-2在结直肠癌组织中的阳性表达率明显高于癌旁组织,差异有统计学意义(P<0.05)。Bcl-2及B7-H1在结直肠癌组织中的表达均与分化程度呈负相关(P<0.01),而且B7-H1在有淋巴结转移组(Dukes分期C+D)与无淋巴结转移组(Dukes分期A+B)相比,阳性表达率升高,差异有统计学意义(P<0.05)。另外,在高-中分化的结直肠癌组织中,B7-H1阴性组Bcl-2阳性表达率明显高于B7-H1阳性组,差异有统计学意义(P<0.01)。短期随访中,死亡的患者均B7-H1表达阳性。结论:B7-H1表达与结直肠癌的侵袭转移及生存期相关,且在高-中分化肿瘤组织中,提示与Bcl-2联合检测可能对结直肠癌的诊断、治疗及预后评价有一定的临床意义。
Objective: To analyze the expression of B7-H1 and Bcl-2 protein in human colorectal cancer, and to explore the relationship between B7-H1 and Bcl-2 protein and clinicopathological features of colorectal cancer. Methods: Immunohistochemistry was used to detect the expression of B7-H1 and Bcl-2 in 57 surgically resected specimens of colorectal cancer and adjacent normal tissues, and the correlation analysis was performed in combination with clinical pathological features. Results: The positive rate of B7-H1 and Bcl-2 in colorectal cancer tissues was significantly higher than that in adjacent tissues. The difference was statistically significant (P<0.05). The expression of Bcl-2 and B7-H1 in colorectal cancer tissues was negatively correlated with the degree of differentiation (P<0.01), and B7-H1 was in the group with lymph node metastasis (Dukes stage C+D) and no lymph node metastasis ( Compared with Dukes stage A+B), the positive expression rate was increased, and the difference was statistically significant (P<0.05). In addition, in high-to-middle differentiated colorectal cancer tissues, the positive expression rate of Bcl-2 in the B7-H1 negative group was significantly higher than that in the B7-H1 positive group, and the difference was statistically significant (P<0.01). During short-term follow-up, all patients who died were positive for B7-H1 expression. Conclusion: The expression of B7-H1 is correlated with the invasion, metastasis and survival of colorectal cancer. It is suggested that the combination of Bcl-2 and Bcl-2 may have a certain value in the diagnosis, treatment and prognosis of colorectal cancer. Clinical significance.