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背景心肌内皮素-1(ET-1)及其受体ETA和ETB在心衰中表达增加。但ET-1及其信号通路在心肌病发病机理中的作用仍不明了。方法和结果将人的ET-1cDNA置于对强力霉素(DOX)调控的转录活化因子(tTA)应答的启动子下游。得到的转基因小鼠(ET+)与心肌特异表达tTA(MHC-tTA)的转基因小鼠杂交
Background The expression of cardiac endothelin-1 (ET-1) and its receptors ETA and ETB is increased in heart failure. However, the role of ET-1 and its signaling pathway in the pathogenesis of cardiomyopathy remains unclear. Methods and Results Human ET-1 cDNA was placed downstream of the promoter responsive to doxycycline (DOX) regulated transcriptional activator (tTA). The resulting transgenic mice (ET +) were crossed with transgenic mice that had cardiac-specific tTA (MHC-tTA)