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目的:胃癌是我国常见的恶性肿瘤,死亡率高,胃癌的治疗仍以化疗为主,而长期化疗使得患者产生耐药,影响化疗效果。中西医结合治疗肿瘤受到关注,本研究将60例患者在术前进行中医辨证,用MTT法测定不同证型患者胃癌细胞体外药物的敏感性并探讨其相关性。方法:将60例原发性胃癌患者手术前进行辨证,术后将癌组织送至实验室提取出胃癌细胞,超低温保存,利用MTT法测定5种化疗药物针对不同中医证型胃癌患者的体外敏感性。并计算其抑制率。抑制率采用均数±标准差(±s)表示,组内数据的比较采用两因素的方差分析,组间两两比较采用重复测量方差分析F检验。当P<0.05具有统计学差异。以上数据均采用SPSS 19.0统计软件处理。结果:5种化疗药物对60例胃癌患者在细胞体外药物敏感性上均无显著性差异(P>0.05)。5种化疗药物在抑制率上气血亏虚组表现为不敏感甚至耐药,两两证型相比气血亏虚组与肝胃不和组以及痰食瘀阻组比较有显著性差异(P<0.05)结论:不同证型胃癌患者对5种化疗药物敏感性有相关性,肝胃不和最敏感,依次为脾胃虚寒、痰食瘀阻,气血亏虚最差。
OBJECTIVE: Gastric cancer is a common malignant tumor in our country with a high mortality rate. Chemotherapy remains the mainstay of gastric cancer treatment. However, long-term chemotherapy results in drug resistance and affects the efficacy of chemotherapy. In this study, 60 patients were treated with TCM syndrome differentiation before operation. MTT assay was used to determine the in vitro drug sensitivity of gastric cancer cells in different syndrome types and its correlation. Methods: Sixty cases of primary gastric cancer patients were diagnosed before operation. The tissues of the patients were collected and sent to the laboratory to extract the gastric cancer cells after cryopreservation. MTT assay was used to determine the sensitivity of the five chemotherapeutic drugs to gastric cancer patients with different TCM syndromes Sex. And calculate the inhibition rate. The inhibition rate was expressed as mean ± standard deviation ( ± s), the data in the group were compared using two-factor analysis of variance, and the two groups were compared by repeated measures analysis of variance ANOVA F test. When P <0.05 was statistically significant. The above data are used SPSS 19.0 statistical software processing. Results: There was no significant difference in vitro drug sensitivity between the five chemotherapeutic drugs and 60 gastric cancer patients (P> 0.05). The five chemotherapeutic drugs were not sensitive or even resistant to the deficiency of qi and blood in the inhibition rate. There was a significant difference between the syndromes of qi and blood deficiency and the syndrome of disharmony of liver and stomach and phlegm and blood stasis group P <0.05) .Conclusion: The gastric cancer patients with different types of syndromes are sensitive to the chemosensitivity of the five chemotherapeutic drugs and are the most sensitive to liver-stomach disharmony, followed by deficiency of spleen and stomach, stagnation of phlegm and blood stasis, and the worst of qi and blood deficiency.