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人们已经从不同种属动物的肠道组织提取液中分离出多种不同氨基酸结构的胆囊收缩素Chole-cystoktnin,简称CCK从猪肠中已经分离出CC-K和CC,并已得出它们的结构式。另一些实验室先后从不同种属动物的肠和脑组织中发现CCK_3。另外狗的CCK_(53)已经被纯化并得到它的结构式。 本文采用900只二周龄的大白鼠肠组织,重613克,按1克加5毫升甲醇的比例搅拌提取、过滤。在滤液中加入巯基乙酸,最终浓度0.1％。放入-20℃
Chole-cystoktnin has been isolated from intestinal tissue extracts of different species of animals and has been isolated from CC-K and CC by porcine intestine and has been derived Structure. Other laboratories have successively found CCK_3 from intestine and brain tissues of different species of animals. In addition the dog’s CCK_ (53) has been purified and its structure is obtained. In this paper, 900 two-week-old white rat intestinal tissue, weighing 613 grams, according to the proportion of 1 gram plus 5 ml of methanol stirring extraction, filtration. Thioglycolic acid was added to the filtrate to a final concentration of 0.1%. Add -20 ° C