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目的：为阐明牙齿发育的机制，探讨程序性细胞死亡（ＰＣＤ）及其相关调控基因在牙齿发育中的作用。方法：利用原位末端标记法、原位杂交及免疫组化技术对ＳＤ大鼠牙齿发育不同时期ＰＣＤ及Ｂｃｌ－２、Ｂａｘ基因的表达进行研究。结果：在牙胚发育早期的生长中心处ＰＣＤ、Ｂｃｌ－２、ＢａｘｍＲＮＡ及其蛋白的表达均很强，牙体组织形成后ＰＣＤ及Ｂｃｌ－２ｍＲＮＡ蛋白的表达逐渐减弱，但Ｂａｘ蛋白的表达仍然明显。结论：Ｂｃｌ－２、Ｂａｘ基因及ＰＣＤ可能是牙齿发育过程中的重要调控因素
Objective: To elucidate the mechanism of tooth development, to explore the role of programmed cell death (PCD) and its related regulatory genes in tooth development. Methods: The expressions of PCD, Bcl-2 and Bax genes in SD rats during different stages of tooth development were studied by in situ end-labeling, in situ hybridization and immunohistochemistry. Results: The expressions of PCD, Bcl-2, Bax mRNA and protein in the early growth phase of tooth germ were very strong. The expressions of PCD and Bcl-2 mRNA decreased gradually after tooth tissue formation, but the expression of Bax protein was still significant . Conclusion: Bcl-2, Bax gene and PCD may be important regulators in tooth development