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人的静脉血中血红蛋白(Hb)-环氧苯乙烯(SO)加合物的分析测定过程是:从人的静脉血中提取的血红蛋白在一定条件下酶解,蛋白质中半胱氨酸残留加合物再经Raney Nickel催化反应生成两种加合物的异构体:α-苯己醇(2-PE),β-苯乙醇(1-PE),再用五氟苯酰氯衍生化,最后用GC/MS测定这两种加合物的含量,同样方法测SD大鼠的血红蛋白-SO加合物(in vitro和in vivo).实验结果表明:在动物实验中,所测的血红蛋白-环氧苯乙烯两种加合物(I-PE,2-PE)与实验的苯乙烯或SO剂量有较好的相关性;且2-PE>1-PE,说明氧化苯乙烯与半胱氨酸的反应α位强于β位.在人群实验中,对一石棉厂职业接触苯乙烯工人(11人)静脉血中的血红蛋白-环氧苯乙烯加合物进行测定,结果是α位的(2-PE),α位+β位(2-PE+1-PE)的加合物与个体接触苯乙烯浓度有较好的相关性;而β位的加合物不明显,实验结果为探索用人的血红蛋白中的半胱氨酸-环氧苯乙烯加合物作为人接触苯乙烯的生物标志物提供了可靠的现场实验依据.
Human venous blood hemoglobin (Hb) - Epoxy styrene (SO) adduct analysis and determination process is: from human venous blood hemoglobin extracted under certain conditions, the protein cysteine residue plus The Raney Nickel catalyst was then used to catalyze the formation of two adduct isomers, α-phenylhexanol (2-PE) and β-phenylethyl alcohol (1-PE), derivatized with pentafluorobenzoyl chloride The contents of these two adducts were measured by GC / MS, and the same method was used to measure the hemoglobin-SO adducts of SD rats (in vitro and in vivo) .Experimental results show that in the animal experiments, the measured hemoglobin- There was a good correlation between the two styrenic adducts (I-PE, 2-PE) and the experimental styrene or SO; and 2-PE> 1-PE, indicating that styrene oxide and cysteine Alpha] is stronger than the [beta] position.In the population experiment, the hemoglobin-epoxidized styrene adduct in venous blood of a workers exposed to asbestos (11) was measured as (2 -PE), α-position + β-position (2-PE + 1-PE) adducts and the individual contact styrene concentration has a good correlation; and β-adduct is not obvious, the experimental results for the exploration of human Hemoglobin in half Cystine-epoxidized styrene adducts provide reliable field evidence for human exposure to styrene.