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鉴定和获取了四种油料作物(油菜、大豆、花生和芝麻)中的细菌型PEPC基因,分析了所编码蛋白的保守结构域(BOX I-IV)和蛋白作用功能位点。基因包括甘蓝型油菜的Bna10093361、Bna1009749和Bna10093360,大豆的Glyma10g34970.1,Glyma01g22840.1和Glyma02g14500.1,芝麻的SIN1018296和花生的AhPPC5。这8个基因通常含有19～21个内含子,内部插入一个约350～600bp的高度变异区,编码的蛋白在C端形成R/KNTG结构域,在N端缺乏磷酸化作用位点。在种子发育的不同时期,油菜中仅Bna10093360表达,但其表达量不到油菜Bn-Actin表达量的0.1%;大豆中Glyma10g34970.1表达量最高(接近大豆GlymaActin的2%),Glyma02g14500.1次之;花生AhPPC5表达量为花生AhActin的32%～175%,在种子不同发育时期表达量为早期>中期>晚期;芝麻SIN1018296表达量为芝麻SINActin的3%～18%,在种子发育时期的表达趋势和花生AhPPC5相似。8个基因种子中的表达模式差异明显,说明细菌型PEPC基因可能存在着广泛的功能分化。
The bacterial PEPC genes in four oilseed crops (rapeseed, soybean, peanut and sesame) were identified and obtained. The functional domains of the conserved domains (BOX I-IV) and protein functions of the encoded proteins were analyzed. Genes include Bna10093361, Bna1009749 and Bna10093360 of Brassica napus, Glyma10g34970.1, Glyma01g22840.1 and Glyma02g14500.1 of soybean, SIN1018296 of sesame and AhPPC5 of peanut. These eight genes usually contain 19-21 introns with a highly variable region of about 350-600 bp inserted. The encoded protein forms the R / KNTG domain at the C-terminus and lacks the phosphorylation site at the N-terminus. At different stages of seed development, only Bna10093360 was expressed in rapeseed, but its expression level was less than 0.1% of the expression level of Bn-Actin in rapeseed. The expression level of Glyma10g34970.1 in soybean was the highest (close to 2% of soybean GlymaActin), Glyma02g14500.1 The expression level of AhPPC5 in peanut was 32% -175% of that of peanut AhActin. The expression level of SINP18 in the peanut was 3% ~ 18% at the stage of seed development The trend is similar to peanut AhPPC5. There were significant differences in the expression patterns among the 8 gene seeds, indicating that there may be a wide range of functional differentiation of the bacterial PEPC gene.