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了解传染性非典型肺炎(severe acute respiratory syndrome,SARS)康复者体内冠状病毒特异性抗体IgG的产生水平及其与肺功能的关系。 方法:抽取305例SARS康复者康复后时期的血液,经病毒灭活后分离血清,用酶联免疫吸附法对冠状病毒特异性抗体IgG进行检测。另选取了180例健康者作为对照。 结果:81.3％(248/305)的康复者IgG抗体为阳性(抗体OD值为1.126±0.337),最长检测到康复165d的患者,该类抗体仍然呈阳性;18.7％(57/305)的康复者IgG抗体为阴性(抗体OD值为0.020 7±0.0264)。180例对照组中该类抗体全部为阴性(抗体OD值为0.019±0.010)。SARS康复者抗体阴性组与健康献血人群组之间不存在显著性差异(t=0.548,P=0.983);而上述两组与SARS康复者抗体阳性组之间存在显著性差异(t=12.321,P=0.000)。 结论:大多数SAPS康复者体内产生了冠状病毒特异的IgG抗体,并且这类抗体可能持续较长时间。
To understand the level of coronavirus-specific IgG production and its correlation with pulmonary function in SARS patients. Methods: Blood was collected from 305 SARS convalescent patients after rehabilitation. Serum was isolated after inactivation by virus and the coronavirus specific IgG was detected by enzyme - linked immunosorbent assay. Another 180 healthy people as a control. Results: 81.3% (248/305) of the recovered patients were positive for IgG antibody (OD = 1.126 ± 0.337), up to 165 days of recovery, the antibodies were still positive; 18.7% (57/305) The recovered IgG antibody was negative (antibody OD was 0.020 7 ± 0.0264). All 180 antibodies in the control group were negative (OD 0.019 ± 0.010). There was no significant difference (t = 0.548, P = 0.983) between the SARS convalescent antibody negative group and the healthy blood donation group. However, there was a significant difference between the two groups and the SARS convalescent antibody positive group (t = 12.321 , P = 0.000). CONCLUSIONS: Most SAPS-positive individuals develop coronavirus-specific IgG antibodies in vivo and these antibodies may persist for longer periods of time.