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用水溶性着色素示踪树液流动法,可诊断早期松枯病症及其恢复现象。接种线虫后一天,在松茎横断面,树脂道周围未着色的白斑扩大。这是树脂扩散后,进入邻近管胞,以及树脂不正常漏出所改。反之,它们又阻碍树脂流动。随病情加剧,白斑越来越明显,侵害面也越来越大,最后形成松脂材。在此阶级,虽然这些扩散了的树脂截断了体内输水系统,水仍会上升到叶部。这时,继而同时出现树脂道、髓射线和髓心薄壁组织坏死现象。水分进入坏死组织后,树液流动就完全不正常。在病症最后阶级,可以观察到,输水系统已完全受阻,接种的松苗中,有些可以看到它们能摆脱病疾而复苏。一些树苗已表明,树茎内部有典型的树脂漏出,它们的形成层都会继续产生正常管胞。这就表明,彻底毁坏形成层,必然会导致松树死亡。上述方法,可以用于诊断松枯萎病。
The use of water-soluble pigment tracer tree fluid flow method can be diagnosed early wilt disease and its recovery. One day after the nematode inoculation, uncolored leukoplakia around the resin road was enlarged in the pine stem cross section. This is after the resin has diffused into the neighboring tube cells, and the resin is not normally leaked. On the contrary, they hinder the resin flow. With the exacerbation of the disease, the white spot is more and more obvious, the noodles are also getting bigger and bigger, and finally the rosin material is formed. At this class, water will rise to the leaf even though these diffused resins cut off the body’s water system. At this time, followed by simultaneous resin Road, medullary ray and parenchyma parenchymal necrosis. After the water enters the necrotic tissue, the sap flow is completely abnormal. In the final stages of the illness, it can be observed that the water delivery system has been completely obstructed and that some of the inoculated pine needles can be seen recovering from illnesses. Some saplings have shown that typical resin leaks inside tree stems and their formation continues to produce normal tracheids. This shows that the complete destruction of the formation will inevitably lead to the death of pine trees. The above method can be used to diagnose pine wilt disease.