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纹状体是中脑黑质多巴胺神经元的主要靶组织，我们先前的研究曾指出：纹状体提取液能增强体外培养的中脑黑质神经元的存活和发育。本研究是在此基础上进一步应用ＳｅｐｈａｄｅｘＧ－７５凝胶层析、高效液相色谱（ＨＰＬＣ）、反相高效液相色谱（Ｒ－ＨＰＬＣ）、ＳＤＳ－ＰＡＧＥ等的分析，结合生物鉴定，从纹状体提取液中分离出纯化的活性因子——纹状体源性神经营养因子。该因子的分子量为１１．５ｋＤ，等电点为９．３９，它能促进体外培养的１４ｄ龄大鼠胚胎中脑黑质神经元的存活和神经突起的生长，以及对外源性多巴胺的摄取，其作用具有量依赖性关系，最适量为２２０μｇ／Ｌ
Striatum is the main target tissue of mesencephalic dopaminergic neurons in our brain. Our previous study pointed out that striatal extract can enhance the survival and development of cultured midbrain substantia nigra neurons in vitro. This study was further based on the analysis by Sephadex G-75 gel chromatography, high performance liquid chromatography (HPLC), reverse phase high performance liquid chromatography (R-HPLC) and SDS-PAGE, Extract from the body extract isolated purified active factor - striatal-derived neurotrophic factor. The molecular weight of this factor is 11.5kD and the isoelectric point is 9.39. It can promote the survival and neurite outgrowth of substantia nigra neurons in midbrain of 14-day-old rat embryos cultured in vitro, as well as the uptake of exogenous dopamine, Its role in a dose-dependent relationship, the optimal amount of 220μg / L