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目的探讨高温暴露对器官形成期胚胎生长发育的影响。方法将15只健康成年未生育清洁级的SD孕鼠随机分为对照组、高温暴露30min组、高温暴露60min组,每组5只。孕鼠于妊娠第8～10天分别暴露于(39.5±0.5)℃的高温环境中。在妊娠第11天检测孕鼠血清中乳酸脱氢酶(LDH)和肌酸激酶(CK)活力及胚胎数、头长和畸形率以及胎鼠脑蛋白质与HSP70含量。结果与对照组比较,高温暴露60min组孕鼠血清中LDH和CK活力均升高,差异有统计学意义(P<0.05)。而高温暴露30min组和对照组孕鼠血清中LDH和CK活力间比较,差异均无统计学意义。随着高温暴露时间的延长,胎鼠头长和脑蛋白质含量呈下降趋势,畸形率和HSP70含量呈上升趋势;但各高温暴露组胎鼠胎鼠头长、畸形率、HSP70含量和脑蛋白质含量间比较,差异均无统计学意义。结论器官形成期孕鼠高温暴露对胎鼠的生长发育产生不良影响。
Objective To investigate the effect of hyperthermia exposure on the growth and development of organ during embryogenesis. Methods Fifteen healthy pregnant SD pregnant rats without reproductive stage were randomly divided into control group, 30-day hyperthermia exposure group, 60-day exposure group, with 5 rats in each group. The pregnant rats were exposed to the high temperature environment of (39.5 ± 0.5) ℃ on the 8th to 10th days of gestation respectively. The activities of lactate dehydrogenase (LDH) and creatine kinase (CK), the number of embryos, the length of head and deformity and the content of HSP70 in fetal rat brain were detected on the 11th day of gestation. Results Compared with the control group, the activities of LDH and CK in serum of pregnant rats exposed to hyperthermia for 60 min were significantly increased (P <0.05). However, there was no significant difference in serum LDH and CK between pregnant rats exposed to hyperthermia at 30 min and control groups. With the prolonged exposure to high temperature, the head length and brain protein content of fetal rats showed a downward trend, while the deformity rate and HSP70 content showed an upward trend. However, the head length, malformation rate, HSP70 content and brain protein content Between the two groups, the difference was not statistically significant. Conclusion The exposure of pregnant rats during organogenesis has adverse effects on the growth and development of fetal rats.