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从产于粤北山区的野生建兰报中分离出4株内生真菌,并对这些真菌在兰根上的感染特征及生理学特性进行了研究。结果表明,菌根真菌多数浸染植株根段离很尖3~18cm的根毛区,而根尖和新长出的根则很少被感染;几乎所有的老根都受过真菌的感染。真菌菌丝从兰报表皮侵入,通过表皮层进入皮层薄壁细胞内部形成菌丝团,而在表皮层并不进入细胞内,也不形成菌丝团。真菌感染极状整后第9天,皮层细胞内的菌丝团开始消解。所分离的菌株能利用大部分简单的有机碳源和氮源,能在营养成分不明确的天然介质中生长良好,但对硝酸盐的利用较差。
Four strains of endophytic fungi were isolated from wild Jianlan reported in the mountainous area of northern Guangdong, and the infection characteristics and physiological characteristics of these fungi on the roots were studied. The results showed that most of mycorrhizal fungi rooted from the root tip of 3 ~ 18 cm from the tip of the root hair, while the root tips and newly grown roots are rarely infected; almost all of the old roots have been fungal infections. Fungal mycelium invades from the blue epidermis and enters the mycelial parenchyma via the epidermis into the cortical parenchyma, whereas it does not enter the epidermis and does not form mycelium. On the 9th day after the fungal infection was extremely positive, the mycelium in cortical cells began to digest. The isolated strains can utilize most of the simple organic carbon and nitrogen sources and grow well in natural media with undefined nutrients but poor utilization of nitrate.