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本文提出了一种基于光电转换纳米复合物(NCs)的新型神经调控方式。NCs是TiO_2纳米晶表面连接金纳米粒子的复合物,可产生光电流并有效引发神经细胞去极化。在405 nm光照射下,NCs产生的光电流比单一TiO_2纳米晶产生的光电流强度显著提高。PC12细胞上的膜电位荧光探针和钙离子荧光探针测试结果显示,在405 nm光照下,经NCs处理的细胞发生去极化。在活体抗癫痫实验中进一步证明,NCs产生的光电流可明显减轻斑马鱼的癫痫发作。本研究结果表明NCs可进行神经调控,对神经疾病的治疗具有重要意义。
In this paper, we propose a novel neuromodulation method based on photoelectric conversion nanocomposites (NCs). NCs are the complexes of gold nanoparticles attached to the surface of TiO 2 nanocrystals, which can generate photocurrent and effectively induce depolarization of nerve cells. Under 405 nm light irradiation, the photocurrent generated by NCs is significantly higher than that of single TiO_2 nanocrystals. The membrane potential fluorescence probe and the calcium ion fluorescent probe on PC12 cells showed that under the light of 405 nm, NCs-treated cells depolarized. In live antiepileptic experiments further evidence that photocurrent generated by NCs can significantly reduce the zebrafish seizures. The results of this study show that NCs can be neuromodulated, and is of great importance to the treatment of neurological diseases.