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化学修饰的免疫球蛋白(Ig)如磺化Ig具有完整IgG分子、明显低的抗外体活性(AKA)以及静注后恢复全部重要的Fc-间接的生物学活性。作者采用Yamanaka等报告的制备硫化Ig的方法及用苏联产化学试剂,在0.15M磷酸缓冲液(pH7.0—7.2)和40—45℃水浴中,对莫斯科HNNЭM产的商品胎盘Ig(浓度5%),按下述三组配比方案进行处理:1,2%亚硫酸钠,24小时;2,2%亚硫酸钠与0.03%硫酸铜,3.5—4.5小时;3.2%亚硫酸钠与0.5—1%四连硫酸钠,3小时。处理后经离心、透析及除菌,成品含2.25%甘氨酸。第1,2组各处理4批,第3组3批,未处理组5批(蛋白浓度10%)。结果AKA(按ГорьковскииНИИЭМ检定静脉注射Ig的方法)以mg蛋白计:未处理≤0.01,试验
Chemically modified immunoglobulins (Ig), such as sulfonated Ig, have intact IgG molecules, significantly lower anti-exosome activity (AKA), and all of the important Fc-indirect biological activities restored after intravenous injection. The authors used the method reported by Yamanaka et al. For the preparation of sulfidic Ig and the commercial placental Ig produced by HNN Chem, Moscow at a concentration of 5 in a phosphate buffered saline (pH 7.0-7.2) and a 40-45C water bath using a Soviet Union- %), According to the following three groups of matching programs: 1,2% sodium sulfite, 24 hours; 2,2% sodium sulfite and 0.03% copper sulfate, 3.5-4.5 hours; 3.2% sodium sulfite and 0.5-1% Sodium, 3 hours. After treatment by centrifugation, dialysis and sterilization, the finished product containing 2.25% glycine. Groups 1 and 2 were treated 4 batches, group 3 batches 3, untreated group 5 batches (protein concentration 10%). Results AKA (method for the determination of intravenous Ig by ГорьковскииНИИЭМ) in mg protein: untreated ≤ 0.01, test