Expression of platelet-derived growth factor-BB in liver tissues of patients with chronic hepatitis

• 作 者: Song-Mei Lou You-Ming Li Kai-Ming Wang Wei-Min Cai Hong-Lei Weng
• 机 构: Department of Gastroenterology, Hangzhou First People’s Hospital, Hangzhou 310006, Zhejiang Province
• 出 处: 世界胃肠病学杂志（英文版）
• 发表日期: 2004年3期
• 关键词: chronic hepatitis B hyaluronic acid liver fibrosis

AIM: To study the relationship between expression of plateletderived growth factor-BB (PDGF-BB) and fibrogenesis in chronic hepatitis B.METHODS: Hepatic tissues from 43 patients with chronic hepatitis B were embedded in paraffin. The sections were stained with HE and picric acid-sirius red to determine inflammatory activity and fibrosis stages. PDGF-BB expression was detected by immunohistochemistry and assessed semiquantatively. Levels of serum hyaluronic acid (HA),pro-collagen Ⅲ (PCⅢ), collagen Ⅳ (Ⅳ-C) and laminin (LN)were examined by radioimmunoassay (RIA).RESULTS: The expression level of PDGF-BB was found to be positively correlated with inflammatory activity, fibrosis stage and grade of histological findings (τ=0.58, 0.55,0.55, P＜0.01). The positive correlation was also observed between tissue level of PDGF-BB expression and contents Of HA, PCⅢ, Ⅳ-C and LN in the circulation (r=0.52, 0.32,0.40, 0.33, P＜0.05).CONCLUSION: PDGF-BB may play some role in the development and progression of liver fibrosis.