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目的探讨石英粉尘对DNA及染色体的损伤作用,为尘肺病防治提供理论依据。方法标准α石英刺激人胚肺成纤维细胞(HELF),采用免疫荧光技术检测H2AX磷酸化(γH2AX)水平以及分析中性彗星实验Olive矩,作为DNA双链断裂(DSBs)损伤程度的指标,微核实验观察染色体损伤情况。结果依次用0、100、200、400μg/mL石英刺激HELF细胞,γH2AX水平呈增高趋势,半定量结果分别为(521.9±233.1)、(823.3±201.5)、(1 375.5±311.5)、(1 545.6±145.7);Olive矩亦呈增高趋势,分别为(1.74±0.24)%、(2.67±0.36)%、(9.23±0.72)%、(14.16±1.05)%,差异均有统计学意义(P<0.05)。相同剂量的石英刺激下,微核率增加,但差异无统计学意义。结论石英粉尘可引起DNA双链断裂,且存在剂量-效应关系。
Objective To investigate the DNA and chromosomal damage effects of quartz dust and to provide a theoretical basis for the prevention and treatment of pneumoconiosis. Methods Standard α-quartz was used to stimulate human embryonic lung fibroblasts (HELF). The levels of phosphorylated H2AX phosphorylated (γH2AX) were measured by immunofluorescence and Olive moment was analyzed as an indicator of DNA damage (DSBs) Nuclear experiment to observe the chromosomal damage. Results HELF cells were stimulated with 0, 100, 200, and 400 μg / mL quartz, and the levels of γH2AX were increased. The semiquantitative results were (521.9 ± 233.1), (823.3 ± 201.5), (1 375.5 ± 311.5), (1 545.6 ± 145.7). The Olive moments also showed a trend of increasing (1.74 ± 0.24)%, (2.67 ± 0.36)%, (9.23 ± 0.72)%, (14.16 ± 1.05)%, respectively, 0.05). Under the same dose of quartz stimulation, micronucleus rate increased, but the difference was not statistically significant. Conclusion Quartz dust can cause DNA double-strand break, and there is dose-effect relationship.