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目的研究益肺解毒方联合顺铂对人肺腺癌A549细胞凋亡的影响。方法应用血清药理学方法制备益肺解毒方药物血清;采用高剂量益肺解毒方药物血清联合顺铂进行凋亡实验;应用Annexin V-FITC/PI双染流式细胞术检测细胞凋亡率,Cell QUEST软件进行分析。结果高剂量益肺解毒方协同顺铂组凋亡率为(39.79±3.21)%,与空白对照组(0.94±0.9)%、顺铂组(20.66±2.70)%比较,P<0.05,有统计学差异,表明益肺解毒方协同顺铂可诱导人肺腺癌A549细胞凋亡并且二者合用作用明显增强。结论益肺解毒方联合顺铂可增强诱导人肺腺癌A549细胞凋亡。
Objective To study the effects of Yifeng Jiedu Decoction combined with cisplatin on the apoptosis of human lung adenocarcinoma A549 cells. Methods Serum pharmacology method was used to prepare the serums of Yifei Jiedu Decoction. The high dose Yiffeji Jiedu Decoction serum combined with cisplatin was used for apoptosis test. The apoptosis rate was detected by Annexin V-FITC / PI double staining flow cytometry, Cell QUEST software for analysis. Results Compared with control group (0.94 ± 0.9)% and cisplatin group (20.66 ± 2.70)%, the apoptosis rate of high-dose yifujiedu group and cisplatin group was (39.79 ± 3.21)%, P <0.05 Learning differences, indicating that yifujiedu side synergistic with cisplatin can induce human lung adenocarcinoma A549 cell apoptosis and combined effect was significantly enhanced. Conclusion Yifu Jiedu Decoction combined with cisplatin can enhance the apoptosis of human lung adenocarcinoma A549 cells.