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目的 探讨硒在机体自然抗肿瘤发生过程中的作用。方法 给断乳雄性 Wistar大鼠 MNNG(N甲基 - N -硝基 - N亚硝胍 2 0 mg/kg)灌胃 ,每天一次 ,连续 1 0 d,以诱导大鼠胃粘膜异倍体形成 (大鼠胃癌模型形成 )。第 2 5wk时 ,用流式细胞仪测定硒对大鼠胃幽门粘膜异倍体形成的影响 ,用免疫组织化学 ABC法观察大鼠胃粘膜异倍体形成过程中 ,其胃的胃泌素细胞 (G细胞 )、生长抑素细胞 (SS细胞 )、5-羟色胺细胞 (5- HT细胞 )的免疫组织化学变化 ,并对以上结果进行定性、定位、定量分析以及统计学处理。结果 1 .硒能抑制 MNNG所致大鼠胃粘膜细胞异倍体的形成。 2 .实验对照组与正常对照组比较 ,大鼠胃粘膜胃泌素细胞的免疫组织化学反应明显增强 (P<0 .0 1 ) ,SS细胞、5- HT细胞也有增强的趋势 (P>0 .0 5)。 3.加硒各组与实验对照组比较 ,大鼠胃粘膜胃泌素细胞的免疫组织化学反应明显减弱 (P<0 .0 1 ) ,SS细胞、5- HT细胞也有减弱的趋势 (P>0 .0 5)。结论 内分泌细胞可能与 MNNG所致大鼠胃粘膜细胞异倍体的形成和发展有关 ;硒可能对大鼠胃内分泌细胞的功能有一定影响
Objective To investigate the role of selenium in the natural anti-tumor process of the body. Methods MNNG (N-methyl-N-nitro-N-nitrosoguanidine 20 mg/kg) was given intragastrically once a day for 10 days to induce aneuploidy in gastric mucosa of weanling male Wistar rats. (rat gastric cancer model formation). At the 25th wk, the effect of selenium on gastric analloplast formation in gastric pylorus mucosa was measured by flow cytometry. Gastric gastrin cells were observed in gastric mucosal aneuploid formation by immunohistochemical ABC method. (G cells), somatostatin cells (SS cells), serotonin cells (5-HT cells), immunohistochemical changes, and the above results were qualitative, localized, quantitative analysis, and statistical processing. Results 1. Selenium can inhibit the formation of aneuploidy of gastric mucosal cells in rats induced by MNNG. 2. Compared with the normal control group, the immunohistochemical reaction of gastric gastrin gastrin cells in the experimental control group was significantly increased (P<0.01), and the SS cells and 5-HT cells also showed an increasing trend (P>0). .0 5). Compared with the experimental control group, the immunohistochemical reaction of gastrin cells in gastric mucosa was significantly decreased (P<0.01), and SS cells and 5-HT cells also decreased (P>0.05). 0 .0 5). Conclusion Endocrine cells may be related to the formation and development of gastric mucosal cell aneuploid cells induced by MNNG; selenium may have a certain effect on the function of gastric endocrine cells in rats.