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目的:建立杏花药材(HPLC)指纹图谱测定方法,对不同产地的杏花药材进行质量评价。方法:采用正交设计优化杏花提取方法,建立HPLC指纹图谱。运用相似度评价、聚类分析与主成分分析相结合的方式对不同产地杏花做出统计分析及质量评价。结果:正交设计最佳提取工艺为70%乙醇20m L超声30min。10批样品共确定15个共有峰,相似度均>0.900,指认了3个特征峰,分别为绿原酸、芦丁、阿魏酸。聚类分析将10批药材聚为4类;主成分分析提取出3个特征峰,其累计贡献率为87.525%。根据3个主成分的综合评价得分判定:10批次样品中来自河北沽源、北京、河北张家口3个产地的山杏花综合得分最高,质量最好。结论:不同产地与基源的杏花药材化学成分相似,但含量存在显著性差异,山杏花较杏花质量好。聚类分析和主成分分析相互补充,互为佐证,与相似度评价三者相结合为杏花药材的质量控制提供依据。
OBJECTIVE: To establish a HPLC fingerprinting method for the determination of apricot flower from different areas. Methods: The orthogonal design was used to optimize the apricot extraction method, and the HPLC fingerprinting was established. Using the method of similarity evaluation, cluster analysis and principal component analysis, statistical analysis and quality evaluation of apricot in different areas were made. Results: The orthogonal design of the best extraction process for 70% ethanol 20m L ultrasound 30min. A total of 15 batches of samples were identified from 10 batches of samples with similarities> 0.900. Three characteristic peaks were identified, which were chlorogenic acid, rutin and ferulic acid respectively. Cluster analysis of 10 batches of herbs into four categories; principal component analysis extracted three peaks, the cumulative contribution rate of 87.525%. According to the three main components of the comprehensive evaluation score to determine: 10 batches of samples from Hebei Guyuan, Beijing, Zhangjiakou, Hebei origin three apricot comprehensive score the highest, the best quality. Conclusion: The chemical constituents of apricot from different habitats and base sources are similar, but the content is significant, and the apricot is better than apricot. Clustering analysis and principal component analysis complement each other, supporting each other, and the similarity evaluation of the three for the quality control of apricot herbs provide the basis.