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甾体皂甙元已在许多种植物的组织培养物中发现。在某些植物的悬浮培养的液体培养基中添加胆甾醇,可使薯蓣皂甙元、提果皂甙元(Tigogenin)和吉托皂甙元(Gitogenin)的含量明显增加,但未能得到海可皂甙元(Hecogenin)。本文研究不同浓度的胆甾醇对威氏剑麻深层培养中形成皂甙元,特别是海可皂甙元的影响。在含有1ppm2,4-D的RT固体培养基上,将威氏剑麻苗诱导的愈伤组织,转移到含有0.1ppm2,4-D的RT液体培养基中进行培养。静置培养物于2,4,6,8
Steroidal saponins have been found in the tissue cultures of many plants. Addition of cholesterol to the liquid culture medium of suspension culture of certain plants can significantly increase the contents of diosgenin, tigogenin and gitogenin, but no saponins can be obtained. (Hecogenin). This paper studies the effects of different concentrations of cholesterol on the formation of saponin, especially the saponins, in deep culture of Wesley sisal. The callus induced by Vibrio sinensis seedlings was transferred to RT liquid medium containing 0.1 ppm 2,4-D on RT solid medium containing 1 ppm of 2,4-D. Resting cultures on 2,4,6,8