论文部分内容阅读
目的:观察丁高大鼠鞘内大麻素水平后实验动物的疼痛行为学变化及脊髓背角星形胶质细胞的激活状态,探讨内源性大麻素参与触诱发痛的可能机制。方法:成功建立大鼠鞘内置管模型后,分别鞘内注射外源性大麻素2-AG、大麻素受体激动剂CP55940、大麻素水解酶抑制剂JZL195。使用Von-frey纤维丝观察给药后不同时间点大鼠机械性缩足反射阈值的变化,运用共聚焦显影观察脊髓背角星形胶质细胞的激活状态。结果:(1)鞘内注射2-AG、CP55940、JZLl95后1 d即产生明显的触诱发痛(P<0.01)并至少持续到给药后第21 d(P<0.001);(2)鞘内注射2-AG、CP55940和JZLl95后第5 d即有明显的星形胶质细胞激活(P<0.01),并可持续到给药后第21 d(P<0.01)。结论:升高鞘内大麻素水平可能通过激活星形胶质细胞导致触诱发痛。
OBJECTIVE: To observe the changes of pain behavior and the activation of astrocytes in spinal dorsal horn of experimental rats after intrathecal cannabinoid levels in rats, and to explore the possible mechanism of endocannabinoids involved in tactile allodynia. Methods: After intrathecal model was successfully established, intrathecal injection of exogenous cannabinoid 2-AG, cannabinoid receptor agonist CP55940 and cannabinoid hydrolase inhibitor JZL195 respectively. Von-frey filaments were used to observe the change of mechanical contracting threshold at different time points after administration, and the activation of astrocytes in spinal dorsal horn was observed by confocal imaging. Results: (1) Tacrolimus induced significant pain (P <0.01) at 1 d after intrathecal injection of 2-AG, CP55940 and JZL195 and continued for at least 21 days after administration (P <0.001) Injecting 2-AG, CP55940 and JZL95 after 5 days showed obvious astrocyte activation (P <0.01), and continued until 21 d after administration (P <0.01). Conclusion: Increasing the level of intrathecal cannabinoid may induce tingling pain through activation of astrocytes.