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目的 :研究三氧化二砷 (As2 O3)对慢性粒细胞K5 6 2 (CML) ,急性粒细胞白血病细胞株HL - 6 0 (AMLM2 )的作用并与细胞株NB4(APLM3)作比较。方法 :采用形态学和流式细胞术观察不同浓度As2 O3 对HL - 6 0 ,K5 6 2及NB4细胞的作用。结果 :As2 O3不仅能诱导NB4的凋亡 ,提高其浓度至 2 .7μM和 8.1μM ,也能诱导K5 6 2 ,HL - 6 0的凋亡。As2 O3 不能诱导三种细胞分化。结论 :As2 O3 对细胞诱导的凋亡是非选择性的且不能诱导分化。
Objective: To study the effect of arsenic trioxide (As2O3) on chronic granulocyte K562 (CML) and acute myelocytic leukemia cell line HL-60 (AMLM2) and compare it with cell line NB4 (APLM3). Methods: Morphological and flow cytometry were used to observe the effects of different concentrations of As2O3 on HL-60, K562 and NB4 cells. Results: As2O3 not only induced apoptosis of NB4, but also increased its concentration to 2. 7μM and 8.1μM. It also induced apoptosis of K562, HL-60. As2O3 cannot induce three kinds of cell differentiation. CONCLUSION: As2O3 is non-selective for cell-induced apoptosis and does not induce differentiation.