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通过对7-乙氧基香豆素O-脱乙基酶(ECOD)和7-甲氧基香豆素O-脱甲基酶(MCOD)在水稻靶标微粒体中活性的测定,获得了磺酰脲类除草剂对水稻中O-脱烷基化作用的选择性和安全作用的基本数据。通过测定烟酰胺腺嘌呤二核苷酸磷酸细胞色素P-450(c)还原酶(NADPH cyt.c.red),证实了电子转移系统对细胞色素P-450反应的影响。安全剂:核酸(NA)和乙醇;抑制剂:1-氨基苯并三唑(1-ABT)和增效醚(PBO);除草剂:哌草丹、1-(α,α-二甲基苄基)-3-(p-甲苯基)脲、吡嘧黄隆(PSE)和苄嘧黄隆(BSM)的使用,控制了细胞色素P-450相关的反应。经过安全剂(NA、乙醇)和除草剂(哌草丹、1-(α,α-二甲基苄基)-3-(p-甲苯基)脲、PSE、BSM)的处理,诱导了O-脱烷基化作用,因此,可通过使用磺酰脲类除草剂(PSE、BSM)和诱导细胞色素P-450酶的抑制剂(1-ABT、PBO)来调节抑制的程度。相对而言,NADPH细胞色素阴极还原酶并没有因受到诱导或抑制作用而发生明显变化。这些结果表明:细胞色素P-450与PSE和BSM磺酰脲类除草剂的氧化代谢机理有关。这些除草剂可以是一个基质或微粒体氧化酶的竞争抑制剂。
By measuring the activity of 7-ethoxycoumarin O-deethylase (ECOD) and 7-methoxycoumarin O-demethylase (MCOD) in rice target microsomes, Basic data on the selective and safe role of ureide herbicides on O-dealkylation in rice. The effect of the electron transfer system on the cytochrome P-450 response was confirmed by measuring nicotinamide adenine dinucleotide phosphate cytochrome P-450 (c) reductase (NADPH cyt.c.red). Safeners: nucleic acid (NA) and ethanol; inhibitors: 1-aminobenzotriazole (1-ABT) and piperonyl butoxide (PBO); herbicides: Benzyl) -3- (p-tolyl) urea, pyrazosulfuron (PSE) and bensilazan (BSM), controlled the cytochrome P-450 related reactions. Treatment with safeners (NA, ethanol) and herbicides (pipermectin, 1- (α, α-dimethylbenzyl) -3- (p-tolyl) urea, PSE, BSM) - Dealkylation, therefore, the degree of inhibition can be modulated by the use of sulfonylurea herbicides (PSE, BSM) and inhibitors of cytochrome P-450 induction (1-ABT, PBO). Relatively speaking, NADPH cytochrome cathode reductase was not induced or inhibited by significant changes. These results indicate that the cytochrome P-450 is involved in the oxidative metabolism of PSE and BSM sulfonylurea herbicides. These herbicides can be a competitive inhibitor of matrix or microsomal oxidases.