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目的:探讨扶正化瘀方(FZHY)对肝纤维化小鼠骨髓细胞肝脏归巢的作用。方法:BABL/C雌性受鼠性别交叉法骨髓移植模型基础上,四氯化碳(CCl4)诱导肝纤维化模型;成模后,治疗组以FZHY方4.6 g.kg-1.d-1 ig 4周。PCR,病理及肝功能检测成模情况;通过FISH法标记Y染色体,观察雌性受鼠肝脏内骨髓源性细胞含量;Western blot法检测雌性受鼠肝组织基质细胞衍生因子-1(SDF-1)蛋白相对表达量。结果:与模型组比较,FZHY组小鼠,肝组织损伤明显减轻。FISH法标记Y染色体观察显示,FZHY组小鼠较模型组肝内骨髓源性细胞减少(P<0.05)。Western blot结果,FZHY组小鼠较模型组肝脏SDF-1蛋白相对表达量明显减少(P<0.01)。结论:在肝脏损伤时,FZHY可能通过抑制肝内SDF-1蛋白的表达来阻止骨髓源性细胞迁移入肝,从而一定程度上抑制骨髓源性细胞入肝后促进肝纤维化。
Objective: To investigate the effect of FZHY on liver homing of bone marrow cells in mice with liver fibrosis. Methods: CCl4-induced hepatic fibrosis model was established on the basis of BABL / C female sex-crossover bone marrow transplantation model. After treatment, FZHY prescription 4.6 g.kg-1.d-1 ig 4 weeks. PCR, pathology and liver function test. The Y chromosome was labeled by FISH, the content of bone marrow-derived cells in the liver of the female mice was observed. Western blot was used to detect the expression of SDF-1, Relative protein expression. Results: Compared with the model group, the FZHY group mice and liver tissue injury was significantly reduced. The FISH staining of Y chromosome showed that the number of bone marrow-derived cells in FZHY group was lower than that in model group (P <0.05). Western blot results showed that the relative expression of SDF-1 protein in FZHY group was significantly lower than that in model group (P <0.01). CONCLUSION: FZHY may prevent bone marrow-derived cells from migrating into the liver by inhibiting the expression of SDF-1 protein in the liver during liver injury, which may inhibit the proliferation of bone marrow-derived cells and promote hepatic fibrosis to a certain extent.