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探讨胆红素在哮喘动物模型建立过程中能否发挥保护作用。通过用卵清蛋白(OVA)对BALB/C小鼠进行致敏、激发建立哮喘模型,并在致敏和激发阶段给予间接胆红素干预。造模成功后通过肺组织病理切片及支气管肺泡灌洗液炎症细胞分类计数观察哮喘模型小鼠的气道炎症程度,ELISA方法测定血清中OVA-sIgE及相关细胞因子IL-4、IFN-γ、IL-2、IL-17、IL-10和TGF-β的水平,免疫组化法检测肺组织IL-17的表达,并检测氧化应激相关指标。研究发现,经胆红素干预后小鼠体内间接胆红素水平升高,肺组织炎症细胞浸润减少,OVA-sIgE和IL-4水平较低而IFN-γ、IL-2和IL-10水平较高,IFN-γ/IL-4增高;肺组织IL-17表达较低,氧化应激反应降低,而对TGF-β的影响较小。可见胆红素在哮喘动物模型建立过程中可通过降低氧化应激反应,促进IL-10的分泌从而调节免疫系统的平衡,改变Th1/Th2/Th17相关细胞因子的分泌格局发挥保护作用。
To explore whether bilirubin can play a protective role in the establishment of asthma animal model. BALB / C mice were sensitized by ovalbumin (OVA) to establish an asthmatic model and were given indirect bilirubin intervention during sensitization and challenge phases. The severity of airway inflammation in asthmatic mice was observed by pathological sections of lung tissue and bronchoalveolar lavage fluid inflammatory cells after successful modeling. The serum levels of OVA-sIgE and related cytokines IL-4, IFN-γ, The levels of IL-2, IL-17, IL-10 and TGF-β were detected by immunohistochemistry. The expression of IL-17 in lung tissue was detected by immunohistochemistry. The results showed that the level of indirect bilirubin in mice increased, the infiltration of inflammatory cells in lung decreased, the levels of OVA-sIgE and IL-4 were lower and the levels of IFN-γ, IL-2 and IL-10 Higher, IFN-γ / IL-4 increased; lower IL-17 expression in lung tissue, decreased oxidative stress, while the impact of TGF-β was small. It can be seen that bilirubin can play a protective role in the animal model of asthma by reducing the oxidative stress reaction, promoting the secretion of IL-10, regulating the balance of immune system and changing the secretion pattern of Th1 / Th2 / Th17 related cytokines.