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目的研究骨桥蛋白(OPN)的表达与黑素瘤浸润、转移的关系。方法采用免疫组化SP法检测OPN在黑素瘤(30例)、黑素细胞痣(30例)、正常皮肤组织(15例)以及在黑素瘤A375细胞株和不同浓度乙酰肝素酶反义寡核苷酸转染的裸鼠黑素瘤移植瘤中的表达。结果 OPN在黑素瘤、黑素细胞痣及正常皮肤组织中阳性表达率分别为86.67%(26/30),13.33%(4/30)和0(0/15),黑素瘤组与黑素细胞痣组及正常皮肤组OPN的阳性表达率比较差异有统计学意义(P=0.000)。3种不同浓度的乙酰肝素酶反义寡核苷酸在转染的裸鼠体内恶性黑素瘤移植瘤中表达表现出不同程度的抑制作用,30μmol/L实验组的抑制作用最强。OPN在人黑素瘤A375细胞株中呈强阳性表达。结论 OPN在黑素瘤中的表达明显高于其在黑素细胞痣和正常皮肤中的表达;乙酰肝素酶反义寡核苷酸对裸鼠体内黑素瘤移植瘤中OPN的表达有显著抑制作用;OPN在A375细胞株中呈强阳性表达。
Objective To study the relationship between osteopontin (OPN) expression and melanoma invasion and metastasis. Methods Immunohistochemical SP method was used to detect the expression of OPN in melanoma (30 cases), melanocytic nevus (30 cases), normal skin tissue (15 cases) and melanoma A375 cell line with different concentrations of heparanase Expression of sense oligonucleotide-transfected nude mouse melanoma xenografts. Results The positive expression rates of OPN in melanoma, melanocytic nevus and normal skin tissues were 86.67% (26/30), 13.33% (4/30) and 0 (0/15), respectively. Melanoma group and black The positive expression rates of OPN in the group of nevus and normal skin were significantly different (P = 0.000). Three different concentrations of heparanase antisense oligonucleotides showed different degrees of inhibition in transplanted malignant melanoma xenografts in nude mice, and the 30 μmol / L experimental group had the strongest inhibitory effect. OPN was strongly positive in human melanoma A375 cell line. Conclusions The expression of OPN in melanoma is significantly higher than that in melanocytic nevus and normal skin. The expression of OPN in hematoma xenograft in nude mice is significantly increased by heparanase antisense oligonucleotide Inhibition; OPN was strongly positive in A375 cell line.