论文部分内容阅读
目的进一步了解c-rel对免疫系统的调控功能特征。方法采用再现差异分析法(representative difference analysis,RDA)分析从野生型和c-rel敲除老鼠所获mRNA。结果发现一个新基因,其表达依赖于完整c-rel,命名为淋巴细胞衍生的C-型凝集素(LCL)-1。LCL-1属Ⅱ型跨膜蛋白,膜外区含一个糖基识别域。该糖基识别域与其他C-型凝集素受体(包括CD69)所携带的糖基识别域具有高度同源性。LCL-1基因位于小鼠6号染色体的NK细胞基因复合体内,编码至少4种可选择的拼接亚型。有趣的是,LCL-1除表达于B细胞,也在不成熟和成熟树突状细胞表达,尤以后者表达水平更高。结论发现一个NKC家族新成员,首次证明NKC家族的凝集素样受体是c-rel的下游基因。
Objective To further understand the regulatory features of c-rel on the immune system. Methods mRNA from wild-type and c-rel knockout mice was analyzed using representative difference analysis (RDA). As a result, a new gene, whose expression is dependent on intact c-rel, was named as lymphocyte-derived C-type lectin (LCL) -1. LCL-1 is a type II transmembrane protein, the outer membrane contains a glycosylation domain. This glycosylation recognition domain has a high degree of homology to the glycosyl recognition domains carried by other C-type lectin receptors, including CD69. The LCL-1 gene is located in the NK cell gene complex on mouse chromosome 6 and encodes at least 4 alternative splicing subtypes. Interestingly, LCL-1 is also expressed in immature and mature dendritic cells, especially in the latter, especially in the latter, in addition to its expression in B cells. Conclusions A new member of the NKC family was discovered. It was first demonstrated that the NKC family lectin-like receptor is a downstream gene of c-rel.