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目的:血清蛋白组学在肿瘤标志物的筛查研究中被广泛应用,本研究采用双向荧光差异电泳联合质谱技术,寻找与维吾尔族妇女宫颈鳞癌相关的血浆蛋白候选肿瘤标志物。方法:收集维吾尔族妇女宫颈病变血浆标本(慢性宫颈炎患者22例,宫颈癌早期患者26例),制备血浆低丰度蛋白质组样品,通过蛋白质双向荧光差异电泳技术分离与鉴别分析,筛选出慢性宫颈炎与宫颈癌早期患者两组间血浆差异表达的蛋白位点,运用质谱和生物信息学技术对其进行鉴定和功能识别分析。结果:确定了宫颈炎和早期宫颈鳞癌患者的血浆低丰度蛋白质组差异荧光电泳图谱,筛选出43个差异表达蛋白位点,通过质谱技术鉴定出16种蛋白质,在宫颈鳞癌早期患者血浆中有7个蛋白上调表达、9个蛋白下调表达。通过生物信息学分析,差异蛋白涉及的通路主要有补体和代谢相关蛋白和酶类。结论:本研究针对新疆维吾尔族妇女宫颈癌及官颈炎患者,展开血浆低丰度蛋白组表达水平的研究,鉴定出多种差异表达蛋白质,为宫颈癌的早期诊断及癌变机制研究提供了依据。
OBJECTIVE: Serum proteomics is widely used in the screening of tumor markers. In this study, two-dimensional fluorescence difference electrophoresis combined with mass spectrometry was used to search for plasma protein candidate tumor markers associated with cervical squamous cell carcinoma in Uighur women. Methods: Uyghur women with cervical lesions (22 cases of chronic cervicitis and 26 cases of early stage cervical cancer) were collected from low-density plasma samples of the Uyghur women. The proteins were separated and identified by two-dimensional electrophoresis Cervical inflammation and early cervical cancer patients between the two groups were differentially expressed plasma protein spots, the use of mass spectrometry and bioinformatics techniques to identify and identify their functions. Results: The difference of plasma low abundance proteome in patients with cervicitis and early cervical squamous cell carcinoma was confirmed by fluorescence electrophoresis, and 43 differentially expressed protein spots were screened. Sixteen proteins were identified by mass spectrometry. In the early stage of cervical squamous cell carcinoma Seven proteins were up-regulated and nine were down-regulated. By bioinformatics analysis, the pathways involved in differential proteins are mainly complement and metabolism related proteins and enzymes. Conclusion: This study aimed to investigate the expression of low-abundance plasma protein in Uygur women with cervical cancer and cervicitis in Xinjiang, and to identify a variety of differentially expressed proteins to provide a basis for the early diagnosis and carcinogenesis of cervical cancer .