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目的观察CT-1C-末端多肽不同干预方式对大鼠心肌缺血再灌注损伤心电图变化的影响。方法用结扎-松解SD大鼠冠状动脉左后降支的方法制作MI/R模型。27只SD大鼠随机分为4组:正常组(N,n=5);疾病组(D,n=6),结扎30min后开始再灌注;MI/R后干预组(T,n=8),结扎30min后开始再灌注并腹腔注射CT-1C-末端多肽100μg/kg;MI/R前干预组(O,n=8),腹腔注射CT-1C-末端多肽100μg/kg后进行MI/R实验。实验过程中,动态监测心电图,观察ST段、心率、PR间期及心律失常的发生情况。结果①结扎前4组大鼠的心率和PR间期与正常组大鼠无差别(F=0.6633,p>0.05);②结扎冠状动脉左后降支30min内,三组大鼠的心率都明显减慢,PR间期也明显延长,与结扎前比较差异有显著性(p<0.01)。再灌注2h内,疾病组(D)大鼠的心率进一步减慢,PR间期也进一步延长,与结扎后比较,差异不显著;MI/R前干预组大鼠的心率及PR间期与结扎后比较,差异有显著性(p<0.01)。MI/R后干预组(T)大鼠心率无明显变化,与结扎后比较,无显著差异;③缺血30min内,仅疾病组(D)有1只大鼠发生心律失常(16.67%);再灌注后,三组大鼠心律失常发生率均有增加,且各组间存在明显差别(x2=4.419,p<0.01),其中T组发生率最低(12.5%),O组发生率最高(62.5%),疾病组(50%)。结论①缺血及再灌注损伤均可导致SD大鼠心率减慢,PR间期延长,并易于再灌注后发生心律失常;②于缺血30min后开始再灌注的同时,施加CT-1C-末端多肽干预,可以防止SD大鼠MI/R后心率进一步减慢,减少心律失常的发生率;③SD大鼠接受CT-1C-末端多肽腹腔注射7天后,再遭受MI/R损伤,其再灌注后的心率进一步明显减慢,且心律失常的发生率增高。
Objective To observe the effects of different interventional modalities of CT-1 C-terminal polypeptide on the changes of electrocardiogram in myocardial ischemia-reperfusion injury in rats. Methods MI / R model was made by ligating - releasing the left posterior descending coronary artery of SD rats. Twenty-seven SD rats were randomly divided into 4 groups: normal group (n = 5), disease group (n = 6), reperfusion after 30min ligation; ), 30 min after ligation, reperfusion and intraperitoneal injection of CT-1 C-terminal polypeptide 100 μg / kg; MI / R pre intervention group (O, n = 8) R experiment. During the experiment, electrocardiogram was monitored dynamically to observe the occurrence of ST segment, heart rate, PR interval and arrhythmia. Results ① There was no difference in heart rate and PR interval between the four groups before the ligation (F = 0.6633, p> 0.05). ② Within 30 minutes after ligation of the left posterior descending coronary artery, the heart rate of the three groups was significantly Slowed down, PR interval was also significantly prolonged, compared with ligation before the difference was significant (p <0.01). Within 2 hours after reperfusion, the heart rate of the disease group (D) was further slowed down and the PR interval was further prolonged, and the difference was not significant after ligation. The heart rate and PR interval in the MI / R pre- After comparison, the difference was significant (p <0.01). There was no significant difference in heart rate between rats in MI / R intervention group (T) and control group (P <0.05). ③In the 30 min ischemia group, only one rat in disease group (D) developed arrhythmia (16.67%); After reperfusion, the incidence of arrhythmia increased in all three groups, with significant difference (x2 = 4.419, p <0.01), of which the lowest incidence was found in T group (12.5%) and the highest in O group 62.5%), disease group (50%). Conclusion ① Both ischemia and reperfusion injury can lead to slowing down of heart rate and prolongation of PR interval in SD rats, and lead to arrhythmia after reperfusion; ② CT-1C-terminal Peptide intervention can prevent SD rat MI / R heart rate further slowed down and reduce the incidence of arrhythmia; ③SD rats received CT-1 C-terminal polypeptide 7 days after intraperitoneal injection, and then suffered MI / R injury, after reperfusion Heart rate further significantly slowed down, and the incidence of arrhythmia increased.