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将SCC－PA／t－PA两种基因CDNA所构建的表达载体PSV_2ScUPA，pcDNA－ScUPA及pcDNA－tPA混合后注入小鼠骨骼肌，观察了基因混合注射在小鼠体内的表述情况。体外凝血试验结果显示．质拉注射后第3天凝血时间开始延长，约两周后凝血时间延长幅度最大。第18天体内诱导产生DIC，体外投血时间试验组仍较空白对照组延长一倍以上．抗凝效果明显。相等基因水平下．混合基因注射表达水平和单独基因注射没有差别。减半注射两种混合基因我体．其抗凝效果与不减量单独注射和混合注射效果相同。
The expression vectors of PSV-2SCUPA, pcDNA-ScUPA and pcDNA-tPA constructed by CDNA of two genes of SCC-PA / t-PA were injected into the skeletal muscle of mice to observe the expression of the gene mixed in mice. In vitro coagulation test results show. On the third day after injection, clotting time began to extend, and the clotting time was extended by about two weeks. On the 18th day, the DIC was induced in vivo, and the time of in vitro priming was longer than that in the blank control group. Anticoagulant effect is obvious. Equal gene level. There is no difference between the mixed gene injection level and the single gene injection. Halo injection of two mixed genes in my body. The anticoagulant effect is the same as the uninjectioned single injection and mixed injection.