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利用正交设计试验法研究了椰乳（ＣＭ）、２，４－Ｄ、赤霉素（ＧＡ３）和蔗糖等四个因素及其组合对石硖龙眼和桂味荔枝的幼胚愈伤组织产生的影响，并讨论了正交设计试验法应用于龙眼和荔枝组织培养培养基筛选的可能性。结果表明：１）对龙眼，诱导愈伤组织的最优组合是 ＣＭ １０％、２，４－Ｄ１．０ｍｇ·１－１、ＧＡ３０ｍｇ·１－１和蔗糖３％；对荔枝，最优组合是ＣＭ０％，２，４－Ｄ１．０ｍｇ·１－１，ＧＡ３０ｍｇ·１－１和蔗糖 ５％。 ２）适当的 ２，４－Ｄ浓度（１．０ ｍｇ·１－１）可提高愈伤组织的产量；３）ＧＡ３抑制了龙眼和荔枝的愈伤组织的产生；４）不同树种的胚需要不同的糖浓度；５）适当ＣＭ浓度（１０％）可提高龙眼愈伤组织的产量，而荔枝愈伤组织的产生并不需要ＣＭ。
Orthogonal design test was used to study the effects of four factors including coconut milk (CM), 2,4-D, GA3 and sucrose on the callus induction of immature embryos , And discusses the possibility of using orthogonal design test method in the screening of longan and litchi tissue culture medium. The results showed that: 1) The best combination of longan and callus induction was CM 10%, 2,4-D1.0mg · 1-1, GA30mg · 1-1 and sucrose 3%; for litchi, the optimal combination was CM0%, 2,4-D1.0 mg · 1-1, GA30 mg · 1-1 and sucrose 5%. 2) Appropriate concentration of 2,4-D (1.0 mg · 1-1) increased the callus yield; 3) GA3 inhibited the callus formation of longan and lychee; 4) embryos of different species needed Different sugar concentration; 5) The appropriate CM concentration (10%) can improve the yield of longan callus, while the callus of litchi does not need CM.