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目的:研究多巴胺转运蛋白(11C-β-CFT)PET成像技术在判别帕金森病(PD)大鼠模型中的作用。材料和方法:22只成功的PD大鼠模型分别进行11C-β-CFTPET显像,ROI方法测量模型大鼠两侧纹状体/小脑比值并进行统计分析,比较两侧有无显著性差异。正常与模型大鼠分别进行TH免疫组化染色。结果:模型大鼠毁损侧纹状体放射性明显下降(P=0.000)。同时模型大鼠TH染色黑质处阳性神经元数量减少。结论:PET多巴胺转运蛋白成像可结合行为学观察作为证实模型成功的检验方法之一,为基础研究及临床诊断PD提供了一种分子成像工具。
Objective: To study the role of dopamine transporter (11C-β-CFT) PET imaging in distinguishing Parkinson’s disease (PD) rat model. MATERIALS AND METHODS: Twenty-two successful PD rats were subjected to 11C-β-CFTPET imaging. ROI was used to measure the striatum / cerebellum ratio on both sides of the model rats and statistical analysis was performed to compare whether there was any significant difference between the two sides. Normal and model rats were immunohistochemically stained for TH. Results: The radioactivity of the injured striatum in the model rats decreased significantly (P = 0.000). Meanwhile, the number of positive neurons in TH-stained substantia nigra of model rats decreased. Conclusion: PET dopamine transporter imaging can be used in combination with behavioral observation as one of the test methods to confirm the success of the model. It provides a molecular imaging tool for basic research and clinical diagnosis of PD.