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Purpose :To study the ability of Homoharringtonine (Hh) ,5-Fluorouracil(5-Fu) , and Adriamycin (ADM) on inhibiting the proliferation of rabbit lens epithelium. Methods : Whole rabbit lenses were removed from freshly enucleated eyes under sterile condition. The rabbit lens eptithlia(RLE) were isolated and culatured: (1) The passage RLE were placed in 24-well tissue culture plates and incubated for 48 hours,then exposed to different concentrations of Hh,5-Fu,and ADM for 24 and 72hours; (2)The passage RLE and Hh(0. 084μg/ml) ,5-Fu(0. 058μg/ml), ADM(0. 45ng/ml)were placed and cultured for 24 hours;(3)The morphological changes of RLE exposed to different concentrations of Hh, 5-Fu and ADM were studied under light microscope.Results: The ID50 of Hh, 5-Fu and ADM exposed to RLE for 24 hours were 0. 84μg/ml,0. 58μg/ml and 4. 50ng/ml,respectively,and those for 72 hours were 0. 49μg/ml,0. 33μg/ml and 3. 85ng/ml.The attachment rate of RLE after being culatured for 24 hours with Hh,5-Fu and ADM were respectin
Purpose: To study the ability of Homoharringtonine (Hh), 5-Fluorouracil (5-Fu), and Adriamycin (ADM) on inhibiting the proliferation of rabbit lens epithelium. Methods: Whole rabbit lenses were removed from freshly enucleated eyes under sterile conditions. The rabbit lens eptithlia (RLE) were isolated and culatured: (1) The passage RLE were placed in 24-well tissue culture plates and incubated for 48 hours, then exposed to different concentrations of Hh, 5-Fu, and ADM for 24 and 72hours; (2) The passage RLE and Hh (0. 084μg / ml), 5-Fu (0. 058μg / ml), ADM (0.45ng / ml) were placed and cultured for 24 hours; Changes of RLE exposed to different concentrations of Hh, 5-Fu and ADM were studied under light microscope. Results: The ID50 of Hh, 5-Fu and ADM exposed to RLE for 24 hours were 0. 84 μg / ml, ml and 4. 50 ng / ml, respectively, and those for 72 hours were 0. 49 μg / ml, 0. 33 μg / ml and 3. 85 ng / ml. The attachment rate of RLE after being culatured for 24 hours with Hh, 5- Fu and ADM were respectin