DNA原位定量分析与膀胱移行细胞癌分级和预后的关系

• 作 者: 夏养志 赵丽珍 王广钧 金阳荪 常沛 温凯胜
• 出 处: 内蒙古医学院学报
• 发表日期: 1994年02期
• 关键词: DNA 膀胱肿瘤 膀胱移行细胞癌 图象分析 积分光密度 脱氧核糖核酸 预后 组织切片 回代 预后估计

用图象分析仪对８６例膀胱移行细胞癌（Ｇ１３３例，Ｇ２２７例，Ｇ３２６例）组织切片进行ＤＮＡ原位定量分析，取得平均灰度、平均积分光密度，２～５倍体以上各区段细胞数及比例，经计算机逐步判别，选取６个参数建立分级判别式，全部病例分级回代结果与ＷＨＯ分级一致率为７６．７４％，其中Ｇ１１００％。Ｇ２５５．６％，Ｇ３６９．２％，对预后的估计也优于单纯形态定量，结果证明ＤＮＡ原位定量分析对膀胱肿瘤的分级判定和预后估计是一个很有用的方法。

The histological sections of 86 bladder transitional cell carcinomas (G133, G227, G326) were analyzed by DNA in situ. The average gray level, the average integral optical density and the cell numbers of 2 ~ 5 ploidy Number and proportion, the gradual determination by computer, select the six parameters to establish the classification discriminant, all cases of the classification and the results of the WHO classification consistent rate was 76.74%, of which G1100%. G255.6%, G369.2%, the prognosis of the prognosis is also better than simple morphometric quantification, the results show that DNA in situ quantitative analysis of bladder cancer grading and prognosis is a very useful method.