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目的 :新型细胞因子白细胞介素(interleukin,IL)-29具有抗病毒、抗肿瘤、免疫调节等作用,但其在破骨细胞分化中的作用尚未见报道。本研究探讨IL-29对核因子κB受体活化因子配基(receptor activation of nuclear-κB ligand,RANKL)诱导小鼠巨噬细胞RAW264.7(破骨前体细胞)向破骨细胞分化的作用。方法:流式检测IL-29特异性受体IL-28Rα在RAW264.7细胞表面的表达,CCK8法检测IL-29对RAW264.7细胞增殖的影响。不同浓度IL-29重组因子加入到RANKL诱导的RAW264.7向破骨细胞分化的体系中,5 d后通过抗酒石酸酸性磷酸酶染色检测IL-29对破骨细胞数目的影响。实时荧光定量PCR检测IL-29对破骨细胞标志性基因TRAP、CTR、CTSK及相关基因TRAF6、RANK表达的影响。结果 :IL-29呈剂量依赖性抑制RANKL诱导的RAW264.7中破骨细胞的形成,100 ng/m L IL-29可显著抑制破骨细胞的形成以及破骨细胞形成相关基因TRAP、CTR、CTSK、TRAF6、RANK的表达。结论 :本研究首次证实IL-29可以抑制破骨细胞形成及其功能。
OBJECTIVE: The new cytokine interleukin (IL) -29 has antiviral, antitumor and immunomodulatory effects, but its role in osteoclast differentiation has not been reported yet. This study was aimed to investigate the effect of IL-29 on osteoclast differentiation induced by receptor activation of nuclear-κB ligand (RANKL) in mouse macrophage RAW264.7 (osteoclast precursor) . Methods: The expression of IL-29 specific receptor IL-28Rα on RAW264.7 cells was detected by flow cytometry. The effect of IL-29 on the proliferation of RAW264.7 cells was detected by CCK8 assay. Different concentrations of IL-29 recombinant factor were added into RANKL-induced RAW264.7 osteoclast differentiation system, and the effect of IL-29 on the number of osteoclasts was detected by tartrate-resistant acid phosphatase staining 5 days later. The effect of IL-29 on the expression of TRAP, CTR, CTSK and TRAF6, RANK in osteoclasts was detected by real-time fluorescence quantitative PCR. Results: IL-29 inhibited the formation of osteoclasts induced by RANKL in RAW264.7 in a dose-dependent manner. IL-29 at 100 ng / mL could significantly inhibit osteoclast formation and TRAP, CTR, CTSK, TRAF6, RANK expression. Conclusion: This study for the first time confirmed that IL-29 can inhibit osteoclast formation and its function.