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目的 探讨刺五加叶皂甙 (ASS)抑制宿主肝癌生长和转移的作用机制。方法 给BABL/c小鼠腹腔内注射HepG2肝癌细胞 ,10d后给予ASS腹腔注射 ,8周后处死小鼠 ,取瘤称重。用免疫组织化学方法检测ASS对移植肝癌组织nm2 3 H1和 p5 3基因蛋白表达的影响及抑瘤率 ,观察ASS对肝癌细胞在小鼠腹腔生长和转移的抑制作用。结果 当ASS剂量为 0 .2 5、1.0 0mg/kg体重时 ,抑瘤率分别为 45 .43%和 72 .0 6 % ,显著低于对照组 (P <0 .0 5 )。ASS 1.0 0mg/kg治疗组的nm2 3 H1阳性表达率明显高于对照组。结论 ASS有抑制人肝癌在小鼠体内生长和转移的作用。其作用随剂量增加而递增 ,此作用可能与ASS促进肝癌细胞nm2 3 H1基因蛋白表达有关。
Objective To investigate the inhibitory mechanism of Acanthopanax senticosus saponins (ASS) in inhibiting the growth and metastasis of host liver cancer. Methods HepG2 cells were intraperitoneally injected into BABL/c mice. After 10 days, HepG2 cells were injected intraperitoneally with ASS. Eight weeks later, mice were killed and the tumors were weighed. Immunohistochemistry was used to detect the effect of ASS on the expression of nm23H1 and p53 gene protein in transplanted hepatocellular carcinoma and the inhibitory rate. The inhibitory effect of ASS on the growth and metastasis of hepatoma cells in the abdominal cavity of mice was observed. Results When the dose of ASS was 0.25, 1.0 mg/kg body weight, the tumor inhibition rates were 45.43% and 72.0%, respectively, which were significantly lower than the control group (P < 0.05). The positive expression rate of nm23H1 in the ASS 1.0 mg/kg treatment group was significantly higher than that in the control group. Conclusion ASS can inhibit the growth and metastasis of human liver cancer in mice. Its effect increases with increasing dose, this effect may be related to ASS to promote nm23H1 gene protein expression in liver cancer cells.