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目的研究1,25-(OH)2D3对1型糖尿病(T1DM)小鼠的作用及可能机制。方法 40 mg/kg STZ腹腔注射C57BL/6小鼠连续5 d建立T1DM模型,5μg/kg 1,25-(OH)2D3隔日腹腔注射,监测血糖、体质量、饮水及饲料消耗,分析各脏器的病理及超微结构。结果 1,25-(OH)2D3治疗14 d后,T1DM小鼠的血糖、饮食、饮水量显著下降(均P<0.05);1,25-(OH)2D3可减轻胰岛炎症状及各脏器的超微结构病变,诱导淋巴细胞的凋亡,并在胰腺中可见典型自噬现象。结论 1,25-(OH)2D3可诱导胰腺细胞的自噬,同时诱导淋巴细胞凋亡,从而减轻T1DM症状。
Objective To investigate the effects and possible mechanisms of 1,25- (OH) 2D3 on type 1 diabetic (T1DM) mice. Methods T1DM model was established by intraperitoneal injection of 40 mg / kg STZ into C57BL / 6 mice for 5 days. The rats were injected intraperitoneally with 5μg / kg 1,25- (OH) 2D3 to monitor blood glucose, body weight, drinking water and feed consumption. Pathology and ultrastructure. Results After the treatment of 1,25- (OH) 2D3 for 14 days, the blood glucose, diet and water intake in T1DM mice were significantly decreased (all P <0.05); 1,25- (OH) 2D3 could reduce the symptoms of insulitis and viscera Of ultrastructural lesions, induce apoptosis of lymphocytes, and typical autophagy seen in the pancreas. Conclusion 1,25- (OH) 2D3 can induce autophagy in pancreatic cells and induce lymphocyte apoptosis, thereby alleviating the symptoms of T1DM.